超声-微波协同提取荷叶总黄酮工艺优化及其提取效果分析  

The optimization on extraction technology of total flavonoids from lotus leaves based on simultaneous ultrasonic/microwave assisted extraction and the analysis of high efficiency

作　　者：付洋[1] 张良慧[1] 江慎华[1,2,3] 杨琼玉[1] 万严 吴真[1] 廖亮[1] 马海乐[1,2] 沈勇根[1,3] 

机构地区：[1]九江学院药学与生命科学学院,江西九江332000 [2]江苏大学食品与生物工程学院江苏省农产品物理加工重点实验室,江苏镇江212013 [3]江西农业大学食品科学与工程学院江西省天然产物与功能食品重点实验室,江西南昌330045

出　　处：《食品工业科技》2015年第18期261-266,共6页Science and Technology of Food Industry

基　　金：国家自然科学基金(31360371);江西省自然科学基金(20132BAB204030);江西省科技支撑计划(20123BBF60150);江苏省农产品物理加工重点实验室开放课题(JAPP2010-5);江西省高等学校大学生创新创业计划;江西省天然产物与功能食品重点实验室开放基金;九江市科技支撑计划(201438)

摘　　要：为优化超声-微波协同提取荷叶总黄酮最佳工艺及解析其提取效率较高的原因,本文通过单因素、正交实验对提取工艺进行优化;通过比较协同提取、超声与微波单独提取及扫描电镜对提取前后荷叶粉末微观结构,初步解释了该技术提取效率较高的原因。结果表明,超声-微波协同提取荷叶总黄酮最佳工艺条件为:提取时间5 min、微波功率125 W、乙醇浓度70%、料液比1∶30 g/m L、超声功率50 W(开)。对比实验发现,在各自最佳提取条件下,协同方法提取荷叶总黄酮效率显著高于水浴振荡提取法(p<0.001);超声与微波单独提取分析发现,协同提取技术结合了超声和微波二者优势、互补产生较高效率提取作用;扫描电镜观察发现,协同提取对原料细胞壁破坏更严重、作用更充分,从而增强了提取效率。超声-微波协同技术对荷叶总黄酮具有较高的提取效率,值得后续深入研究与开发。The objective of the study was to optimize extraction technology of ultrasonic/microwave assisted extraction（UMAE） and to explain the reason for high-efficiency of this technology. The technology optimization was completed by the single factor experiment and orthogonal experiment of L9（ 34）. The reason for high-efficiency was explained by the comparison experiment,splitting analysis and scanning electron microscopy（SEM）. The results of the optimal parameters of UMAE technology were the extracting time 5 min,the microwave power 125 W,the concentration of ethanol 70%,the solid-liquid ratio 1∶30 g/m L and the ultrasonic power 50 W（open）. Comparison experiment indicated that the extraction ratio of total flavonoids from lotus leaf by UMAE was significantly higher than that of water bath shaking extraction（ WBSE） under both optimum conditions（p〈0.001）. It was also found that the highly efficient extraction of UMAE was simultaneously acted and mutually reinforced by ultrasonic and microwave by the splitting analysis. The materials and cell walls were more seriously destroyed so as to achieve higher extraction efficiency. UMAE was a extraction technology with high efficiency and worth of further research and development.

关 键 词：荷叶 总黄酮 超声微波协同提取 扫描电镜 

分 类 号：TS201.1[轻工技术与工程—食品科学]