响应面法优化草鱼皮中有效抗氧化物质提取工艺  

Optimization of enzymolysis conditions for antioxidant components from Grass Carp Skin by response surface methodology

作　　者：吴晓洒 蔡路昀[1,2] 黄作庆 苏洵[1] 冷利萍 宋卓[1] 励建荣[1,2] 

机构地区：[1]渤海大学食品科学与工程学院,生鲜农产品贮藏加工及安全控制技术国家地方联合工程研究中心,辽宁省食品安全重点实验室,辽宁锦州121013 [2]西南大学食品科学学院,重庆北碚400716 [3]大连天宝绿色食品股份有限公司,辽宁大连116600

出　　处：《食品工业科技》2015年第19期202-206,212,共6页Science and Technology of Food Industry

基　　金：国家自然科学基金(31401478;31471639);中国博士后基金面上项目(2015M570760);"十二五"国家科技支撑计划(2012BAD29B06);辽宁省科技攻关项目(201500098)

摘　　要：以草鱼皮为原料,采用六种蛋白酶酶解,以DPPH自由基清除率和水解度为指标,筛选两种较优的酶,再通过响应面分别优化两种酶制备抗氧化酶解液的工艺条件,并在此基础上进行双酶酶解实验,确定酶解草鱼皮的最优工艺。结果表明:碱性蛋白酶酶解草鱼皮的最适条件为加酶量6.3%、料水比1∶40.6、温度51.3℃、时间115.1 min,此时清除率最高为54.98%。木瓜蛋白酶最适条件为加酶量4.6%、料水比1∶41.2、温度50.5℃、时间133.0 min,清除率最高为41.06%。采用分步酶解(先碱性蛋白酶后木瓜蛋白酶)得到的酶解液DPPH自由基清除能力最高为92.07%,明显优于单酶水解和混合酶解的效果。Six kinds of proteases were used to hydrolyze grass carp skin in order to select two optimum proteases by determining DPPH radical scavenging capacity and degree of hydrolysis（ DH）.Response surface methodology （RSM） was used to optimize hydrolysis conditions of two optimum proteases.Based on the results of RSM,the double enzyme hydrolysis tests were carried out to find out the best hydrolysis conditions.The results showed that hydrolysis conditions for alcalase were enzyme addition amount of 6.3%, material-liquid ratio of 1：40.6, hydrolysis temperature of 51.3 ℃,and hydrolysis time of 115.1 min,and the scavenging activity on DPPH free radicals under the above conditions reached 54.98%.The optimal hydrolysis conditions for papain were 4.6%, 1：4].2,50.5 ℃ and 133.0 min,and the scavenging activity reached 41.06%.By combined and sequential use of alcalase followed by papain,the DPPH scavenging activity was 92.07% ,which was more effective than either of them.

关 键 词：草鱼皮 响应面 酶解 提取 抗氧化 

分 类 号：TS254.9[轻工技术与工程—水产品加工及贮藏工程]