HPLC法测定单糖酸水解体系中5-HMF的研究  

HPLC method for determination of 5- HMF in monosaccharide acid hydrolysis system

作　　者：刘淑兰[1] 余筱洁[1] 刘斌[2] 赵静[1] 叶晓非[1] 马海乐[1] 周存山[1] 

机构地区：[1]江苏大学食品与生物工程学院,江苏镇江212013 [2]中国农业机械化科学研究院,北京100083

出　　处：《食品工业科技》2015年第19期304-307,384,共5页Science and Technology of Food Industry

基　　金：国家自然科学基金项目(31170672);国家博士后基金特别资助和面上资助(2014T70489;2013M541621);江苏省博士后基金项目(1302152C);江苏高校优势学科建设工程资助项目(PAPD);江苏大学高级人才启动项目(12JDG074);江苏大学青年学术带头人培育(2012)资助

摘　　要：本研究建立了直接进样测定葡萄糖、果糖和柠檬酸、磷酸的糖酸高温体系中5-HMF含量的高效液相色谱法,采用安捷伦Lc1260高效液相色谱仪,紫外检测器,检测波长284 nm,流动相为5%（V/V）甲醇水溶液。经过测定条件分析（检测波长、流动相比例、流速和样品过滤方式）与方法评价（选择性、精密度、检出限、回收率和样品测定等）研究,得到标准曲线方程为y=0.006x-0.137,相关系数R2为0.999,精密度高,样品回收率在98%-102%之间。在测定样品中的5-HMF含量时,样品溶液测定的目的峰附近基本无杂峰。In this study,5- HMF determination was carried out by high performance liquid chromatography in monosaccharide- acid catalytic system with direct injection.The optimization of the determination was approached using Agilent Lc1260 HPLC and UV detector.The detection wavelength was 284 nm and the mobile phase was 5%（ 5 /95,v/v） methanol deionized water solution.By the analysis of measurement conditions（ detector wavelength,mobile phase ratio and velocity,filter ways） and evaluation（ Selectivity,accuracy,detection limit,recovery rate,etc）,the standard curve equation was y = 0.006x-0.137,the correlation coefficient R2 was 0.999.The accuracy was high and the sample recovery rate was from 98% to 102%.The proposed methodology was applied to analysis of5- HMF successfully in samples. The analytical performance of the method made it suitable for the routine determination of samples.The sample solution measured out pure peaks and there were no impure peaks.

关 键 词：5-HMF 糖酸体系 高效液相色谱法 准确度 精确度 

分 类 号：TS207.3[轻工技术与工程—食品科学]