小黑药总黄酮抗氧化性研究  

Antioxidant Activity of Total Flavonoids from Sanicula astrantiifolia Wolff

作　　者：杨涛[1] 韩磊[1] 冯国强[1] 宋培培[1] 刘品华[1] 

机构地区：[1]曲靖师范学院化学化工学院,云南曲靖655011

出　　处：《现代农业科技》2015年第17期311-313,316,共4页Modern Agricultural Science and Technology

基　　金：云南省大学生创新创业训练计划项目;上海师范大学资源化学省部共建教育部重点实验室项目;曲靖师范学院资源与环境化学重点实验室项目(SYS2010BX01)

摘　　要：研究小黑药全株总黄酮的含量及提取分离,并用Schaal烘箱法、紫外光照法测试了小黑药总黄酮和对照品BHT抗猪油氧化的能力,采用POV抑制率评价了抗氧化能力。测试了总黄酮和芦丁的还原能力、抑制超氧阴离子自由基(O2-·)的能力、DPPH自由基的清除能力、羟基自由基的清除能力。结果表明:小黑药总黄酮有良好的抗猪油氧化的作用,其抗光氧化的效果高于自动氧化;还原能力、抑制超氧阴离子自由基(O2-·)的能力、DPPH自由基的清除能力略低于芦丁,羟基自由基的清除能力高于芦丁;小黑药全株总黄酮含量为2.52%。In this paper,the content and the optimum extraction of total flavonoid from Sanicula astrantiifolia Wolff were studied.The antioxidant activity of total flavonoids and the reference substance BHT were detected by Schaal Oven Method and UV photo-induced,and the antioxidant activity was estimated by inhibition ratio of POV .The reduction capacity of total flavonoids and Rutin,the inhibition ratio of total flavonoids to superoxide free radical（O2-·）,its DPPH and hydroxyl free radicals-cavenging effect were determined.The results showed thatThe total flavonoids from Sanicula astrantiifolia Wolff showed significant inhibitory effects on the oxidation of lard oil,its antioxidant effect was greater than automatic oxidation.The reduction capacity,the inhibition ratio to superoxide free radical（O2-·）,its DPPH free radicals-cavenging effect were slightly lower than Rutin,and its hydroxyl free radicals-cavenging effect was greater than Rutin.The content of total flavonoid from Sanicula astrantiifolia Wolff was 2.52%.

关 键 词：小黑药 总黄酮 抗氧化性 过氧化值 

分 类 号：TS202.3[轻工技术与工程—食品科学]