耐酸耐高温α-淀粉酶及其菌种选育研究进展  

Research progress in the acid-resistant thermostable α-amylase and strain breeding

作　　者：尹伊[1] 屈建航[1] 李海峰[1] 焦国宝 丁长河[1] 屈凌波[1] 刘仲敏 

机构地区：[1]河南工业大学生物工程学院,河南郑州450000 [2]河南仰韶生化工程有限公司,河南渑池472400

出　　处：《粮油食品科技》2015年第5期101-105,共5页Science and Technology of Cereals,Oils and Foods

基　　金：国家科技支撑计划项目(2013AA102101);国家自然科学面上基金项目(31370147);河南省高校科技创新团队支持计划(15IRTSTHN019);河南省高校青年骨干教师资助计划(2013GGJS-077)

摘　　要：耐酸耐高温α-淀粉酶能在高温与低p H下水解淀粉,是重要的新型工业酶制剂。概述了耐酸耐高温α-淀粉酶的热稳定性、p H稳定性及金属离子对其的影响,阐述了产酶菌种的主要来源现状,并重点对耐酸耐高温α-淀粉酶诱变育种的复合诱变、基因工程定点突变等菌种选育和古菌的相关研究进行了综述。The acid -resistant and thermostable α - amylases is one of the important industrial enzyme preparation, which can hydrolyze starch under high temperature and low pH. The influence of thermal stability, pH stability and metal ions on the acid -resistant and thermostable α -amylases was outlined. The main strain resources were summarized. The research progress in strain improvement and archaea, including compound mutation of mutation breeding and gene engineering site -directed mutagenesis, was discussed.

关 键 词：耐酸耐高温α-淀粉酶 酶学性质 菌种 选育 基因工程 

分 类 号：TQ925.1[轻工技术与工程—发酵工程]