丙酮酸钠与流加发酵结合对唾液酸产量的影响  

Influence of Sodium Pyruvate Combined with Fed-batch Fermentation on Yield of Sialic Acid

作　　者：李春[1] 王敏[1] 戚晓熙[1] 孙金威 刘丽波[1] 刘宁[1,2] 

机构地区：[1]东北农业大学食品学院乳品科学教育部重点实验室,哈尔滨150030 [2]东北农业大学食品学院乳品科学教育部重点实验室,哈尔滨150030

出　　处：《中国食品学报》2015年第7期154-159,共6页Journal of Chinese Institute Of Food Science and Technology

基　　金：“十二五”国家科技支撑计划项目(2011BAD09B0304)

摘　　要：利用微生物发酵法生产唾液酸,凭借其原料价格低廉,唾液酸收率高,产品质量稳定,易于实现连续化生产等优势而备受关注。探讨不同含量的前体物质丙酮酸钠对大肠杆菌K235发酵产唾液酸的影响,以及结合流加发酵进一步提高终产物产量的策略。以唾液酸质量浓度和菌体干质量为参数,通过单因素试验和一次性补料方法分别确定丙酮酸钠的最佳添加量和最佳补料期。试验结果表明,用三口烧瓶模拟发酵罐,在发酵初期添加4 g/L的丙酮酸钠,其发酵液经酸水解后,唾液酸产量达到4.10 g/L。用3.7 L自动生物发酵罐验证,唾液酸产量达到5.24 g/L,比不添加丙酮酸钠时唾液酸的产量提高了146%。丙酮酸钠在发酵8 h时一次性补料,发酵罐中唾液酸的产量达到6.47 g/L,比在单一的分批培养模式中添加丙酮酸钠提高了23.5%。The fermentation technique for sialic acid production has attracted more and more attention due to a lower price of raw material and a higher recovery rate of sialic acid for stable quality, which can be easily applicated in continuous production. The aim of our study is to investigate the effect of different content of sodium pyruvate, precusor substance, on ferment of Escherichia coli K235 to produce sialic acid and further improve the yield of end-product by combining with fed-batch fermentation. Sialic acid concentration and dry cell weight are used for parameters, the optimized quantity of sodium pyruvate and the optimized feeding period was confirmed by single factor experiment method and one-time feeding, respectively. Experimental results showed that adding 4 g/L of sodium pyruvate at the beginning of fermentation and using flask-3-neck simulate fermentor, sialic acid yield reached to 4.10 g/L after acid hydrolysis of fermentation production was performed. 3.7 L automatic biological fermentation tank used to further confirme the final yield of sialic acid can reach to 5.24 g/L, increasing by 146% as compared with fermentation production without sodium pyruvate. Sodium pyruvate combined with one-time feeding culture in at 8 h, sialic acid yield reached to 6.47 g/L in fermentation tank, increasing by 23.5% as compared with adding sodium pyruvate in single batch fermentation.

关 键 词：丙酮酸钠 唾液酸 产量 流加发酵 

分 类 号：TQ920.6[轻工技术与工程—发酵工程]