产原儿茶酸北五味子内生细菌WF17的鉴定及抑菌活性研究  

Studies on the Identification of Protocatechuic Acid-producing Endophytic Bacterium from Schisandra chinensis and Its Antibacterial Activity

作　　者：张萍[1] 吴丹[1] 矫佳陈琦 郑春英[1] 

机构地区：[1]微生物黑龙江省高校重点实验室黑龙江大学生命科学学院,哈尔滨150080

出　　处：《中国食品学报》2015年第7期198-202,共5页Journal of Chinese Institute Of Food Science and Technology

基　　金：黑龙江省自然科学基金面上项目(D200812);黑龙江省博士后科研启动金项目(LBH-Q06016);黑龙江省教育厅海外学人科研资助项目(1154h02);黑龙江省中医药科研立项项目(ZHY08-W78)

摘　　要：研究北五味子内生细菌WF17及其次生代谢产物,旨在获得有良好抑菌活性物质的生产方法。从北五味子果实中分离得到1株对12种受试致病菌具有较强抑制活性的内生细菌WF17,通过对其形态特征观察、生理生化特性鉴定及16S rDNA序列同源性分析,鉴定该内生细菌为枯草芽孢杆菌(Bacillus subtilis)。利用薄层层析法及高效液相色谱方法对WF17的发酵液进行分析,试验结果表明,该菌发酵液中含有原儿茶酸。WF17的获得可为原儿茶酸成分的开发利用提供新途径。To investigate the endophytic bacterium named WF17 which was isolated from the fruit of Schisandra chinensis, and its secondary metabolites. It inhibited effectively the growth of the 12 pathogens with a good antibacterial ability. It was identified as Bacillus subtilis by the 16S rDNA sequence homology and its physiological and biochemical characteristics. Thin-layer chromatography （TLC） and HPLCanalysis indicated that WF17 could produce protocatechuic acid, which provided a new means for development and application of protocatechuic acid.

关 键 词：北五味子 内生细菌 抑菌活性 枯草芽孢杆菌 原儿茶酸 

分 类 号：TQ460.1[化学工程—制药化工] TS201.3[轻工技术与工程—食品科学]