菜籽粕直接发酵和生物预处理后发酵产伊枯草菌素A性能比较研究  

Comparison of iturin A production performance by direct fermentation of rapeseed meal and fermentation after bio- pretreatment of rapeseed meal

作　　者：李坤朋[1] 张欣然[1] 黄凤洪[1] 金虎[1] 钮琰星[1] 陈守文[1] 

机构地区：[1]中国农业科学院油料作物研究所,油料脂质化学与营养湖北省重点实验室,武汉430062

出　　处：《中国油脂》2015年第10期54-58,共5页China Oils and Fats

基　　金：国家自然科学基金(31201461);国家支撑计划(2011BAD26B01-3);948计划(2011-G8(4)-1)

摘　　要：以一株产伊枯草菌素A的枯草芽孢杆菌为研究对象,对比考察了菜籽粕直接发酵和生物预处理后发酵伊枯草菌素A的表达差异,在此基础上进一步考察了不同碳氮源对伊枯草菌素A表达的影响。研究结果表明:菜籽粕经黑曲霉和米曲霉固态发酵及自溶预处理后,游离氨基氮质量浓度得到大幅提升,其发酵起始质量浓度分别是菜籽粕直接发酵的8.7倍和5.6倍;菜籽粕直接发酵下伊枯草菌素A的表达水平明显高于经黑曲霉和米曲霉预处理后发酵的水平,最高质量浓度达0.69 g/L,且高于以商业化蛋白胨和酵母粉作为氮源时的最高水平;相对于其他可溶性碳源而言,低水溶性缓释碳源麸皮和玉米皮更有利于伊枯草菌素A的表达,当以麸皮作为碳源时,其最高质量浓度达到0.96 g/L,是葡萄糖作为碳源时的1.6倍。The comparison of expression differences between direct fermentation of rapeseed meal and fer- mentation after bio - pretreatment of rapeseed meal to produce iturin A by Bacillus subtilis was studied. Then the effect of different carbon and nitrogen sources on the expression of iturin A was further investiga- ted. The results showed that solid state fermentation of rapeseed meal by Aspergillus niger and Aspergillus oryzae with cell autolysis could greatly increase mass concentration of free amino nitrogen, and its initial mass concentrations were 8.7 times and 5.6 times as many as direct fermentation of rapeseed meal, re- spectively. The expression level of iturin A produced by direct fermentation of repeseed meal was signifi- cantly higher than that produced by fermentation of repeseed meal after AspergiUus niger and Aspergillus oryzae treatments, and the highest mass concentration of iturin A reached 0. 69 g/L, which was higher than the highest level with commercial peptone and yeast powder as the nitrogen source. Compared with other soluble carbon sources, wheat bran and corn bran which were low water soluble and release carbonsources were more beneficial to iturin A. When using wheat the expression of bran as carbon source, the highest mass concentration of iturin A reached 0. 96 g/L, 1.6 times as many as that using glucose as carbon source.

关 键 词：菜籽粕 伊枯草菌素A 液态发酵 枯草芽孢杆菌 麸皮 

分 类 号：TS229[轻工技术与工程—粮食、油脂及植物蛋白工程] S482[轻工技术与工程—食品科学与工程]