不同前体物质的添加对高山被孢霉生长及脂肪酸组分的影响  

Effect of different precursors on the growth and fatty acid components of Mortierella alpine

作　　者：王海军[1,2] 杨恒星 王迪[1,2] 陈树林[2] 贾士儒[1] 李德茂[2] 

机构地区：[1]天津科技大学生物工程学院,天津300457 [2]中国科学院天津工业生物技术研究所,天津市工业生物系统与过程工程重点实验室,天津300308 [3]通标标准技术服务(天津)有限公司,天津300457

出　　处：《中国油脂》2015年第10期68-73,共6页China Oils and Fats

基　　金：国家863计划(2014ARA021701);国际科技合作与交流专项(2014DFA61040);天津专项(12ZCZDSF02000,13ZCZDSY05500)

摘　　要：通过添加植物油及吐温作为高山被孢霉合成油脂的前体物质,分别考察了不同前体物质的添加对菌体的生物量、油脂含量、油脂产量及所得油脂中各脂肪酸组分的影响。实验结果显示:添加植物油作为前体不但可以提高菌体中油脂含量,而且会使油脂中花生四烯酸的产量得到大幅度提高,其中加入菜籽油、亚麻籽油及大豆油为前体时,花生四烯酸的产量分别达到了2 063.2、2 062.8 mg/L及2 192.9 mg/L,比对照组提高了112.72%、112.68%及126.10%;而以吐温80为前体时,虽然菌体的油脂产量有所降低,但油脂中花生四烯酸的产量比对照组提高了79.91%,达到了1 744.9 mg/L。The effects of different precursors on the biomass, the content, productivity and fatty acid com- ponents of Mortierella alpine oil were investigated by adding different kinds of vegetable oils and tween as the precursors of Mortierella alpine to produce oil. The results indicated that both the oil content and the productivity of arachidonic acid increased by adding vegetable oil as precursor. When seed oil and soybean oil were added as precursors, the productivity of arachidonic acid reached 2 063.2, 2 062.8 mg/L and 2 192.9 mg/L, increasing by 112.72% ,112.68% and 126.10% compared with the control group. When Tween 80 was used as the precursor, the productivity of Mortierella alpine oil de- creased, while the productivity of arachidonic acid reached 1 744.9 mg/L, increasing by 79.91% com- pared with the control group.

关 键 词：高山被孢霉 油脂 脂肪酸组分 花生四烯酸 

分 类 号：TQ920.1[轻工技术与工程—发酵工程] TQ645.6