产普鲁兰酶海单胞菌的分离鉴定及发酵条件优化  

Isolation and identification of pullulanase producing strain and optimization of its fermentation conditions

作　　者：严芬[1] 杨光[1] 连燕萍 王培松[1] 吴晨烁 

机构地区：[1]福州大学生物科学与工程学院,福建福州350116

出　　处：《食品与发酵工业》2015年第9期40-44,共5页Food and Fermentation Industries

基　　金：海洋公益性行业科研专项(201305015)

摘　　要：以普鲁兰糖为唯一碳源,利用平板涂布法从盐湖中分离到1株产普鲁兰酶的细菌YH-6,结合菌株形态特征和16S rRNA基因序列分析,确定该菌株为海单胞菌(Oceanimonas sp.)。通过单因素试验对发酵培养基以及发酵条件进行优化,优化后的培养基组分(g/L)为:玉米淀粉15.0,酵母膏10.0,KH_2PO_41.0,FeSO_4·7H_2O0.01,NaCl 26.5,MgCl_2·7H_O 2.1,KCl 0.9,CaCl_·H_2O 1.2,MgSO_4·7H_2O 4.2,pH 7.0;最佳培养条件为:发酵温度30℃,转速200 r/min,发酵时间为36 h。优化后的普鲁兰酶的活力可达1.85 U/mL,比优化前的1.28 U/mL提高了44.53%。A novel pullulanase-producing strain YH-6 was isolated from the salt lake using pullulan as the sole carbon source. Based on the morphological observation and 16S rRNA sequence analysis, the strain was identified as Oceanimonas sp. The optimum fermentation medium was composed of 15 g/L corn starch, 10 g/L yeast extract, 1.0 g/L KH2PO4, 0.01 g/L FesO4 · 7H2O, 26.5 g/L NaCl, 2.1 g/L MgCl2 · 7H2O, 0.9 g/L KCl, 1.2 g/L CaCl2· H2O, 4.2 g/L MgSO4 · 7H2O. Under the optimum culture conditions of initial pH 7.0, 30 % and shaking speed of 200 r/min for 24 h, the highest enzyme activity of pullulanase could reach 1.85 U/mL, which was improved by 44.53%.

关 键 词：普鲁兰酶 海单胞菌(Oceanimonas sp.) 鉴定 发酵条件 

分 类 号：TQ925[轻工技术与工程—发酵工程]