HS-SPME-GC/MS对比分析四棱豆叶和花中挥发性成分研究  

Contrastive Analysis of Volatile Components in Leaves and Flowers of Psophocarpus tetragonolobus( Linn. ) DC.

作　　者：张伟[1] 朱晓娣[2] 张娟娟[1] 张勇[1] 尹震花[1] 

机构地区：[1]黄河科技学院,郑州450063 [2]河南大学中药研究所,开封475004

出　　处：《天然产物研究与开发》2015年第10期1732-1736,共5页Natural Product Research and Development

基　　金：河南省高等学校青年骨干教师资助计划(2013GGJS-220);郑州市科技局科技攻关计划(20120684;20140790);河南省科技厅重点科技攻关(142102310147);河南省教育厅高等学校重点科研项目(15A310022)

摘　　要：首次采用顶空固相微萃取四棱豆叶和花挥发性成分,气相色谱-质谱联用技术检测其成分,面积归一化法计算各成分的相对含量,并进行比较分析。结果显示,从四棱豆叶挥发油中鉴定出32个化学成分,占挥发油总量的65.60%,其主要成分为十七烷(5.75%)、2-甲基-十六烷(5.29%)、姥鲛烷(5.05%)、3-甲基-十六烷(4.26%)、棕榈酸(3.76%)、2-溴-十二烷(3.14%)、六氢法尼基丙酮(2.95%)、二氢猕猴桃内酯(2.89%)和肉豆蔻醚(2.59%)等,主要由烷烃类(38.25%)、酮类(8.49%)、酸类(3.76%)、酯类(6.76%)和醚类(3.51%)组成;四棱豆花中共鉴定出33个化学成分,占挥发油总量的76.91%,其主要成分为棕榈酸(11.42%)、六氢法尼基丙酮(10.77%)、十七烷(4.79%)、十六烷(4.69%)、肉豆蔻醚(3.88%)、姥鲛烷(3.42%)、2-溴-十二烷(3.10%)、3-甲基-十六烷(2.97%)、油酸(2.50%)和己酸(2.43%),主要由烷烃类(29.42%)、酮类(13.29%)、酸类(19.51%)、酯类(5.89%)和醚类(4.71%)组成。两者共有成分23个,种类与种数存在一定差异,且其相对含量也存在明显差异,这些成分赋予了肉豆蔻花和叶药理作用的差别。The volatile components in leaves and flowers of Psophocarpus tetragonolobus （ IAnn. ） DC. were extracted by headspace solid phase microextraction for the first time, and their chemical components were identified by GC-MS and relative content of the components were calculated with peak area normalization method. The results showed that 32 com- pounds,representing 65.60% of the total of volatile compounds,were identified from the leaves. The main components were heptadecane （5.75%） ,2-methyl-hexadecane （5.29%）, pristane （5.05%）, 3-methyl-hexadecane （4.26%）, palmitic acid （ 3. 76% ）, 2-bromo dodecane （ 3. 14% ）, hexahydrofarnesylacetone （ 2. 95% ）, dihydroactinidiolide （2. 89% ） and myristicin （ 2.59% ）, and was mainly composed of alkanes （ 38.25% ）, ketanes （ 8.49% ）, acids （3.76%）, esters （6. 76% ） and ethers （3.51%）. 33 compounds were identified from flowers, which comprised 76. 91% of the volatile fraction. The main components were plamitic acid （ 11. 42% ）, hexahydrofarnesylacetone （ 10.77% ）, heptadecane （4.79%）, hexadecane （4.69%）, myristicin （ 3.88% ）, pristane （3.42%）, 2-bromo dode- cane （3.10%） ,3-methyl-hexadecane （2.97%） ,oleic acid （2.50%） and hexanoic acid （2.43%）, and was mainly composed of alkanes （29.42%）, ketones （ 13.29% ）, acids （ 19.51% ）, esters （5.89%） and ethers （4.71%）. 23 compounds were common in both parts of P. tetragonolobus with different quantities. These ingredients gave different pharmacological activity between leaves and flowers of P. tetragonolobus.

关 键 词：四棱豆 叶 花 挥发性成分 顶空固相微萃取 气相色谱-质谱 

分 类 号：R284.2[医药卫生—中药学]