肠炎沙门氏菌gDNA标准物质的研究  

Study on the Reference Materials of Salmonella enteritidis Nucleic Acid

作　　者：柯璐[1,2] 黄晓蓉[1,2] 林杰[1,2] 戴晓丽[1,2] 曾维扬[1,2] 张温玲[1,2] 

机构地区：[1]福建出入境检验检疫局,福建福州350001 [2]福建省检验检疫技术研究重点实验室,福建福州350001

出　　处：《食品与生物技术学报》2015年第8期891-895,共5页Journal of Food Science and Biotechnology

基　　金：福建省科学技术厅社会发展重点项目(2011Y0002)

摘　　要：作者对制备肠炎沙门氏菌核酸标准物质(g DNA)进行初探,共制备200支样品,每支约含有肠炎沙门氏菌g DNA 1μg。方差分析结果显示,F值为0.835 7,小于F0.05(14,30)=1.68,表明在95%显著性水平时,样品的g DNA含量均匀;稳定性试验表明样品在-20℃下保存12个月,g DNA含量维持在1μg/支,0.8 g/d L的琼脂糖凝胶电泳的目标条带清晰,以inv A基因为引物的PCR反应产物电泳条带清晰,样品均具有较好的稳定性。The preparation of nucleic acid reference materials of Salmonella Enteritidis（g DNA） was carried out in this study. A total of 200 samples were prepared,each of them contained 1 μg of Salmonella Enteritidis g DNA. Variance analysis results showed that F value was 0.835 7F0.05（14,30） = 1.68,indicating that g DNA con ple was evenly in the 95% significance level. Stability tests showed that g DNA content of the samples was maintained at 1 μg/support after stored for 12 months at the temperature of-20 ℃. The goal electrophoresis band of 0.8 g/d L agarose gel and the electrophoresis bands of PCR reaction product by using the inv A gene as primers were clear revealed that the property of samples were stable.

关 键 词：肠炎沙门氏菌 核酸 标准物质 

分 类 号：TS207.4[轻工技术与工程—食品科学]