响应面法优化超声提取木槿花多酚的工艺研究  

Ultrasonic- assisted extraction technology optimization of polyphenols from Hibiscus syriacus L.

作　　者：张雪娇[1] 金晨钟[1,2] 胡一鸿[1,2] 谭显胜[1] 何丽芳[1] 王艳[1] 陈勇[1,2] 

机构地区：[1]湖南人文科技学院农业与生物技术学院,湖南娄底417000 [2]湖南省2011协同创新中心,湖南娄底417000

出　　处：《食品工业科技》2015年第21期228-231,236,共5页Science and Technology of Food Industry

基　　金：湖南省教育厅项目(13C442);湖南省自然科学基金(12JJ6019);湖南省高校产业化培育项目(13CY030);国家大学生创新性实验项目(201410553005;2014-502)

摘　　要：以木槿花为原料,利用响应面法对提取木槿花多酚的工艺条件进行优化。在单因素实验的基础上,以超声功率、超声时间、料液比、乙醇体积分数为影响因素,利用Box-Behnken中心组合方法进行4因素3水平实验设计,以多酚得率为响应值进行响应面分析。结果表明,最优提取条件是:超声功率112 W,时间49 min,料液比1∶36(g/m L),乙醇体积分数69%,在此条件下木槿花多酚得率达20.507 mg/g,与理论值20.518 mg/g相近,比用乙醇溶液浸提的多酚得率提高12.91%;超声波辅助法提取木槿花多酚简便、提取率高,回归模型合理可靠,可用于实际预测。The ultrasonic- assisted extraction of polyphenols from Hibiscus syriacus L. was optimized by using response surface methodology.According to the previous study of single variable test,an ultrasonic procedure for the extraction of polyphenols from H. syriacus L.was established. A three- level- four- factor test was employed in this study to optimize the factorial parameters（ ultrasonic power,ultrasonic time,ratio of solvents to raw material,and ethanol concentration） by employing Box- Behnken design（ BBD） of response surface methodology,and the polyphenols yield was taken as the response of the designed experiments. The results showed that the optimal extraction conditions for the extraction of polyphenols from H. syriacus L. were ultrasonic power 112 W,ultrasonic time 49 min,ratio of solvents to raw material 1∶36（ g / m L）,and ethanol concentration 69%,respectively.Under these conditions,the yield of H. syriacus L.polyphenols was 20.507 mg / g,close to the theoretical value 20.518 mg / g.Extraction rate of the ultrasonic assisted extraction of polyphenols from H.syriacus L.was increased by 12.91% as compare to the ethanol extraction.The study revealed that this ultrasonic- assisted methodology,which was simply and high efficient,was suitable for extraction of H.syriacus L.polyphenols,and the regression model was reliable for the yield prediction in practice.

关 键 词：木槿花 多酚 响应面 超声辅助提取 

分 类 号：TS201.1[轻工技术与工程—食品科学]