茶皂素纯化方法的比较及其组分的鉴定  

Comparison of purification methods and component identification of tea saponin

作　　者：张海龙[1] 张维农[2] 蒋继丰[1] 杜静[1] 杨凡[3] 

机构地区：[1]怀化学院生命科学系,民族药用植物资源研究与利用湖南省重点实验室,湖南怀化418008 [2]武汉轻工大学食品科学与工程学院,武汉430023 [3]国家粮食局武汉研究设计院有限公司,武汉430079

出　　处：《中国油脂》2015年第11期94-98,共5页China Oils and Fats

摘　　要：为了获得能用于茶皂素组分分析及鉴定的高纯度茶皂素,采用有机溶剂沉淀法、乙醇重结晶法、大孔树脂法和反相柱层析法4种方法对冷榨油茶籽饼中的茶皂素进行纯化,以茶皂素紫外吸收特征峰强度为指标,比较4种方法的纯化效果。结果表明:反相柱层析法能有效去除茶皂素粗品中大部分杂质,是获得高纯度茶皂素的有效方法,得到的茶皂素样品纯度为95%,可作为茶皂素的标准品。利用高效液相色谱和质谱对茶皂素反相柱层析纯化物进行组分分析,验证了其中5种组分为文献中已经报道的茶皂素单体。The organic solvent precipitation method, ethanol recrystallization method, macroporous resin method and reversed phase column chromatography method were used to purify tea saponin prepared from cold pressed oil - tea camellia seed cake so as to obtain high purity tea saponin for the analysis and iden- tification of tea saponin components. The purification effects of the four methods were compared with vio- let characteristic absorption band intensity as indicator. The results showed that the reversed phase col- umn chromatography method was an effective way to remove most of the impurities and obtained high puri- ty tea saponin. The purity of prepared tea saponin sample reached 95 % , which could be used as tea sap- onin standard. The component analysis of tea saponin sample obtained by reversed phase column chroma- tography method by HPLC and MS indicated that the structures of five components were the same as those in previous report of tea saponin monomers.

关 键 词：茶皂素 纯化 组分 标准品 高效液相色谱 质谱 

分 类 号：TQ423.2[化学工程]