24孔板法诱变选育高产红曲黄色素菌株  

Breeding of Monascus with high-yield yellow pigment by 24 well-plate assay combining with mutation

作　　者：杨强[1,2] 岳建明[1] 王成涛[1,2] 文雁君 

机构地区：[1]北京工商大学,北京市食品添加剂工程技术研究中心,北京100048 [2]北京工商大学,食品质量与安全北京实验室,北京100048 [3]河南中大生物工程有限公司,河南郑州451162

出　　处：《食品工业科技》2015年第22期156-159,共4页Science and Technology of Food Industry

基　　金：国家自然科学基金青年基金项目(31401669,31301411);北京市科技计划项目(Z151100001215008);北京市科技计划项目;北京市属高等学校食品科学创新团队项目(IDHT20130506)

摘　　要：以红曲霉F9-2为出发菌株,24孔板培养法结合紫外线-氯化锂复合诱变处理选育高产红曲黄色素菌株。实验结果表明:在紫外照射130 s、氯化锂添加浓度为0.1%的复合诱变条件下得到的菌株Y16,经液态发酵后产黄色素高达488 U/m L,与出发菌相比,黄色素色价提高了37.1%。经10次传代培养,色价稳定,可作为生产红曲黄色素的优良菌株。Monascus F9-2 was used as the original strain;then the high-yield yellow pigment of Monascus Y16 strain was obtained by UV（130 s） and Li Cl（0.1%） complex mutation combining with the 24 well-plate assay.The results showed that the yellow pigment value of Monascus Y16 could reach 488 U/m L and the value was37.1% higher than the starting strain. The stable genotype was verified through ten times of transferring of culture and was hence a fine strain for producing high-yield yellow pigment.

关 键 词：红曲霉 24孔板 复合诱变 红曲黄色素 

分 类 号：TS201.3[轻工技术与工程—食品科学]