高产褐藻胶裂解酶菌株的筛选及发酵条件优化  

Screening of alginate lyase-producing strains and optimization of fermentation conditions

作　　者：严芬[1] 连燕萍 杨光[1] 王培松[1] 吴晨烁 陈宁辛 

机构地区：[1]福州大学生物科学与工程学院,福建福州350116

出　　处：《食品工业科技》2015年第22期287-292,共6页Science and Technology of Food Industry

基　　金：海洋公益性行业科研专项资助项目(201305015)

摘　　要：采用透明圈初筛方法得到一株产褐藻胶裂解酶菌株B1,通过形态观察和16S r DNA序列分析鉴定该菌株为假交替单胞菌属(Pseudoalteromonas sp.)。通过单因素实验对菌株B1产酶条件进行优化,最佳培养基组成为:褐藻酸钠1%,氯化铵0.2%,Na Cl 3%,KH2PO40.02%,Mg SO40.01%,Ca Cl2·2H2O 0.1%,初始p H5.5。最佳的培养条件:装液量75 m L,培养温度30℃,摇床转速180 r/min,培养22 h。在该条件下,褐藻胶裂解酶最高酶活力提高到71.94 U/m L,提高了72.11%。A bacterial alginate lyase-producing strain B1 was screened with transparent circle method,It was identified as Pseudoalteromonas sp. according to its morphological observation and 16 S r DNA sequence analysis to identified strain B1.And by single-factor method to optimize its enzyme production conditions,the optimal medium component were,sodium alginate 1%,NH4 Cl 0.2%,Na Cl 3%,KH2PO40.02%,Mg SO40.01%,Ca Cl2·2H2O 0.1%,initial p H5.5,the optimal culture conditions were as follows ：75 m L medium in 250 m L Erlenmeyer flask,Cultured at 30 ℃,shaking speed of 180 r/min,for 22 h. Under the optimal culture conditions,the highest enzyme activity of alginate lyase was up to 71.94 U/m L,improving 72.11%.

关 键 词：褐藻胶裂解酶 假交替单胞菌 筛选 鉴定 培养条件 

分 类 号：TS201.3[轻工技术与工程—食品科学]