响应面法优化苹果渣生产细菌纤维素培养基及产物性能研究  

Optimization of medium for bacterial cellulose fermentation with pomace by response surface methodology and property studies of the product

作　　者：张雯[1] 李宏杰 刘兰[1] 齐香君[1] 

机构地区：[1]陕西科技大学食品与生物工程学院,陕西西安710021 [2]华东医药集团,浙江杭州311000

出　　处：《食品工业科技》2015年第23期228-233,共6页Science and Technology of Food Industry

基　　金：陕西省科技厅自然基金项目(2012JM2005);陕西省教育厅科研专项项目(15JK1108);2013年国家级大学生创新创业训练计划项目(201310708014);西安市科技计划项目(CXY1513(3))

摘　　要：为提高木醋杆菌(Acetobacter xylinum)发酵苹果渣水解液生产细菌纤维素(Bacterial cellulose,BC)的产量,采用响应面法对发酵培养基进行优化,同时利用傅里叶红外光谱(FT-IR)和X-射线衍射(XRD)对发酵产物BC的性能和结构进行比较。单因素及响应面实验结果确定木醋杆菌(Acetobacter xylinum)发酵苹果渣水解液生产BC的最佳培养基配方为:蔗糖38.44 g、蛋白胨10.91 g、硫酸镁0.85 g、黄嘌呤0.87 g、乙醇10 m L、苹果渣水解液1000 m L、p H6.0,在此条件下BC的产量为7.19 g/L,较优化前(5.65 g/L)提高了27.3%。苹果渣水解液发酵产物BC结构性能与基本培养基发酵产物BC基本一致。说明苹果渣能够替代部分发酵原料发酵生产BC,且不影响BC性能。In order to increase the bacterial cellulose （BC） productivity of Acetobacter xylinum using pomace hydrolysate, response surface methodology was used to optimize the fermentation medium. Meanwhile, the properties of BC were compared through fourier transform infrared（FT-IR）and X-ray diffraction（XRD）.According to the results of single factor experiments and response surface experiments, the optimal fermentation medium were determined as follows： saccharose 38.44 g, peptone 10.91 g, magnesium sulfate 0.85 g, xanthine 0.87 g, ethanol ]0 mL, pomace hydrolysate 1000 mL and pH6.0, under these conditions,the BC productivity was 7.19 g/L, increased by 27.3% in cemparision to before（5.65 g/L）.The properties and structures of BC produced with pomace hydrolysate were confirmed to be basically same with BC produced with basic medium,which indicated that the pomace hydrolysate could be used as part of raw materials to ferment BC and would not affect the properties of BC.

关 键 词：苹果渣 细菌纤维素 培养基优化 响应面法 

分 类 号：TS201.1[轻工技术与工程—食品科学]