壳聚糖对果蝇生长发育及抗氧化能力的影响  

Effects of chitosan on growth and anti-oxidant capacity of Drosophila melanogaster

作　　者：刘梅[1] 郑美[1] 史挺 

机构地区：[1]枣庄学院生命科学学院,山东枣庄277160 [2]北京资源公司,北京102600

出　　处：《食品工业科技》2015年第24期349-351,355,共4页Science and Technology of Food Industry

摘　　要：为了探讨壳聚糖对果蝇生长发育及抗氧化能力的影响。在果蝇基础培养基中分别加入0.01%、0.05%、0.10%壳聚糖,检测其对果蝇的寿命、抗氧化酶活力及抗氧化相关调控基因表达的影响。结果表明,壳聚糖各剂量组的果蝇半数死亡数与对照组比较,差异不显著(p>0.05);而0.05%、0.10%壳聚糖剂量组的果蝇平均寿命、最高寿命天数高于对照组(p<0.05)。添加各剂量组壳聚糖均可以显著升高GSH-Px活性(p<0.05),给予0.05%和0.10%壳聚糖可以显著升高SOD酶活力(p<0.05),且降低MDA水平(p<0.05)。与对照组相比,壳聚糖处理的0.05%组和0.10%组,果蝇的Cu ZnSOD、Mn-SOD、CAT、PHGSH-Px基因表达均有显著上升的趋势(p<0.05)。综上,壳聚糖能够促进果蝇的生长发育,显著提高果蝇体内抗氧化酶系的活性及其基因表达水平,增强果蝇抗氧化能力,延缓果蝇衰老。The experiment was conducted to determine the effects of chitosan on growth performance and antioxidant capacity of Drosophila melanogaster. The research was focus on the effect of supplied with different doses（0%,0.01%,0.05%,0.10%） of chitosan on Drosophila melanogaster life span,antioxidant enzymes activities and their encoding genes expressions. The results showed that LD50 of Drosophila melanogaster had no difference between treatment groups,compared with control group（p〈0. 05）. Supplying with 0.05%,0.10%chitosan could increase average life-span and maximum life-span of Drosophila melanogaster significantly（p〈0. 05）. Chitosan could increase GSH-Px activity significantly（p〈0. 05）. Supplying with 0.05%,0.10% chitosan could increase SOD activity significantly（p〈0. 05） of Drosophila melanogaster,while the content of MDA was decreased（p〈0. 05）. Real Time-PCR assay results showed that the m RNA expression of Cu Zn-SOD,Mn-SOD,CAT and PHGSH-Px（p〈0. 05） were increased. In summary,the results showed that chitosan had a promotional function on lengthening growth and development,improving the activity of anti oxidative enzymes and gene expression level significantly,enhancing the antioxidant capacity and postponing senility of Drosophila melanogaste.

关 键 词：壳聚糖 黑腹果蝇 寿命 抗氧化能力 

分 类 号：TS201.4[轻工技术与工程—食品科学]