凝胶渗透色谱-气相色谱-串联质谱测定食用油中邻苯二甲酸酯  

Determination of phthalate esters in edible oil by gel permeation chromatography and gas chromatography- tandem mass spectrometry

作　　者：李万平 曹九超[2] 金俊[2] 金青哲[2] 王兴国[2] 

机构地区：[1]中粮东海粮油工业(张家港)有限公司,江苏张家港215634 [2]江南大学食品学院食品营养与安全协同创新中心,江苏无锡214122

出　　处：《中国油脂》2015年第12期62-65,共4页China Oils and Fats

摘　　要：研究建立了食用油中5种邻苯二甲酸酯（PAEs）含量的凝胶渗透色谱-气相色谱-串联质谱（GC-MS／MS）测定方法。将食用油用环己烷-乙酸乙酯（体积比1：1）溶解，凝胶渗透色谱分离纯化后进GC-MS／MS检测。结果表明：邻苯二甲酸二乙基酯（DEP）、邻苯二甲酸二异丁基酯（DIBP）、邻苯二甲酸二丁基酯（DBP）、邻苯二甲酸丁基苯甲基酯（BBP）、邻苯二甲酸（2-乙基己基）酯（DEHP）多通道扫描的最佳碰撞电压分别为2l、23、21、7,9V；5种PAEs的平均加标回收率在80．52％～98．30％之间，相对标准偏差在0．39％～7．62％之间；仪器检出限小于1．0μg/L，方法检出限小于50．0μg/kg。The method for determining the contents of five kinds of phthalate esters （PAEs） in edible oil by gel permeation chromatography and gas chromatography - tandem mass spectrometry （ GC - MS/MS） was studied and established. The edible oil was dissolved in eyclohexane - ethyl acetate （ volume ratio 1 ： 1 ）, separated and purified by gel permeation chromatography, and determined by GC - MS/MS. The results showed that the impact voltages of multi - channel scanning of diethyl phthalate （ DEP）, di - isobutyl phthalate （ DIBP）, dibutyl phthalate（ DBP）, butylbenzyl phthalate （BBP） and di - （2 - ethyl hexyl） - phthalate （DEHP） were 21,23, 21, 7 V and 9 V, respectively. The average recoveries of standard addi- tion for five kinds of PAEs were between 80.52% and 98.30%, and the relative standard deviations were between 0.39% and 7.62%. The limits of detection of the instrument and the method were less than 1.0 μg/L and 50.0 μg/kg, respectively.

关 键 词：食用油 邻苯二甲酸酯 凝胶渗透色谱 气相色谱一串联质谱 

分 类 号：O657.63[理学—分析化学] TS227[理学—化学]