体外模拟消化过程中大豆蛋白的亚基组成及分子质量分布  

Subunit Composition and Molecular Weight Distribution of Soybean Protein in Vitro Simulated Digestion Process

作　　者：江连洲[1] 王瑞[1] 李秋慧[1] 隋晓楠[1] 王中江[1] 齐宝坤[1] 张巧智[1] 李杨[1] 

机构地区：[1]东北农业大学食品学院,哈尔滨150030

出　　处：《中国食品学报》2015年第10期65-72,共8页Journal of Chinese Institute Of Food Science and Technology

基　　金：黑龙江博士后科研启动金(LBH-Q13018);黑龙江省教育厅科学技术研究项目面上项目(12531049);黑龙江省青年科学基金(QC2013C014);大豆产业技术体系岗位专家(CARS-04-PS25)

摘　　要：采用胃蛋白酶对大豆分离蛋白进行体外模拟消化,随着消化时间的延长,大豆蛋白质的消化降解程度逐渐增大,在消化2-5 h内水解度整体变化趋势平稳,消化5 h时,水解度达到8.36%。采用十二烷基硫酸钠-聚丙烯酰胺凝胶电泳对不同的消化条件下的大豆分离蛋白消化产物的亚基结构进行表征,试验结果表明,大豆蛋白的不同组分受胃蛋白酶的消化或降解行为存在明显差异,胃蛋白酶对不同蛋白组分的消化情形与其空间结构紧密相关。采用分子筛凝胶葡聚糖Superdex 200和Sephadex G-75对天然的大豆分离蛋白的分子质量分布进行表征及测定,结果表明,天然的大豆分离蛋白的分子质量范围在980670-2526 u,随着消化反应的不断进行,大豆蛋白质的消化产物分子质量分布主要集中在3 000-10000 u之间,还有小部分小于3000 u的蛋白质及肽存在,该研究旨在更好地了解胃蛋白酶体外模拟大豆分离蛋白过程中大豆蛋白的解离机制。Using pepsin in vitro digestion of soybean protein isolated, With the extension of digestion time, the soybean protein digestion degradation degree increases gradually. In the digestive 2-5 h, DH smooth change in the overall trend, digestion 5 h, DH reached 8.36%. Subunit structure of different digestion conditions of soybean separation protein digestion products were characterized, using twelve sodium dodecyl sulfate polyacrylamide gel electrophoresis（SDS-PAGE）, Experimental results show that, different groups of soybean protein was obvious difference in pepsin digestion or degradation behavior, Pepsin digestion of the situation and its spatial structure of different protein components are closely related. Using molecular sieve gel glucan Superdex 200 and Sephadex G-75 of natural soy protein on characterization and determination of the distribution of molecular weight, The experimental results show that the natural range of the molecular weight of soybean protein isolated in 980670-2526 u, With the ongoing digestive reactions, soybean protein digestion product molecular weight distribution of concentrated between 3 000-10 000 u, there exists a small portion is smaller than 3 000 u proteins and peptides The aim of this study was to better understand the mechanism of soybean protein dissociation process simulation of soy protein isolate in pepsin in vitro simulated digestion.

关 键 词：大豆蛋白 体外模拟消化 胃蛋白酶 亚基组成 分子质量分布 

分 类 号：TS201.21[轻工技术与工程—食品科学]