氮源和无机盐对2株凝结芽孢杆菌L-乳酸发酵影响的研究  

Effects of nitrogen source and inorganic salt on the L-lactic acid production of two Bacillus coagulans strains

作　　者：郑兆娟[1,2] 林茜[1] 臧颖[1] 陈菲菲[1] 欧阳嘉[1,3] 

机构地区：[1]南京林业大学化学工程学院,南京210037 [2]江苏省生物质绿色燃料与化学品重点实验室,南京210037 [3]省部共建林木遗传与生物技术重点实验室,南京210037

出　　处：《生物学杂志》2015年第6期55-59,共5页Journal of Biology

基　　金：国家自然科学基金(31300487;31200443);江苏省杰出青年基金(BK2012038);江苏省自然科学基金(BK20130970);江苏省高校优势学科建设工程资助项目(PAPD)

摘　　要：以2株优良的L-乳酸生产菌株凝结芽孢杆菌XZL4和凝结芽孢杆菌NL01为研究对象,从降低培养基原料成本的角度出发,考察了5种有机氮源、2种无机氮源以及4种无机盐对凝结芽孢杆菌发酵过程的影响。实验结果表明,酵母粉是最优氮源,但不同菌株对酵母粉的依赖程度不一;磷酸二氢钾可以有效促进L-乳酸的生产,适量磷酸二氢钾的添加甚至可以取代部分酵母粉的作用,极大地降低了培养基成本。Bacillus coagulans is a wonderful L-lactic acid producer. In this study, two B. coagulans strains, B. coagulans NL01 and XZL4, were taken as examples to investigate the effects of nitrogen source and inorganic salt on L-lactic acid production. In order to re-duce the cost of culture media, 5 kinds of organic nitrogen, 2 kinds of inorganic nitrogen, and 4 kinds of inorganic salt were selected to investigate their effects on fermentation. The results showed that yeast exact was the best nitrogen source, but its effects on B. coagu-lans NL01 and XZL4 were different. Compared with previous reports, the most exciting result was that potassium dihydrogen phosphate could intensively improve the concentration of L-lactic acid even instead of partial yeast extract. And this would distinctly reduce the cost of culture media.

关 键 词：凝结芽孢杆菌 有机氮源 无机氮源 无机盐 L-乳酸 

分 类 号：TQ921.3[轻工技术与工程—发酵工程]