气相色谱法检测饮料中二羰基化合物  

Detection of Dicarbonyl Compounds in Beverages by Gas Chromatography

作　　者：王晨[1] 李晓明[1] 卢永翎[1] 郑铁松[1] 吕丽爽[1] 

机构地区：[1]南京师范大学金陵女子学院,江苏南京210097

出　　处：《食品科学》2015年第24期235-241,共7页Food Science

基　　金：江苏省基础研究计划(自然科学基金)资助项目(BK2012850)

摘　　要：建立同时在线检测丙酮醛和乙二醛的气相色谱方法。确定检测二羰基化合物的最佳条件为:以丁二酮为内标,以邻苯二胺为衍生化试剂,邻苯二胺的用量67倍于二羰基化合物、衍生化时间10 min、萃取溶剂二氯甲烷、超声时间15 min、萃取2次、柱箱初始温度40℃、程序升温5℃/min,色谱柱载气流量2.0 m L/min,分流比1∶1。丙酮醛和乙二醛的定量限(RSN≈10)分别为0.06 mg/L和0.08 mg/L,检出限(RSN≈ 3)分别为0.02 mg/L和0.03 mg/L,方法灵敏度高。This study established an efficient method for the detection of methylglyoxal （MGO） and glyoxal （GO） with a gas chromatograph （GC）. The optimum experimental conditions were set as follows： ratio of O-phenylenediamine to a-dicarbonyl compounds, 67; derivatization time, 10 rain; extraction solvent, methylene chloride; ultrasonic extraction time, 15 min; and the number of extraction, 2. The injector was operated in split mode with a split ratio of 1：1, and the carrier gas （helium） flow rate through the chromatographic column was 2.0 mL/min. The GC oven temperature was programmed as follows： the initial oven temperature was set as 40 ℃ and then increased at a rate of 5 ℃/min. The minimum quantitation limits （RSN approximately equal to 10） and detection limits （RSNS approximately equal to 3） for MGO and GO were 0.06 and 0.08 mg/L, and 0.02 and 0.03 mg/L, respectively. This method was sensitive and efficient.

关 键 词：丙酮醛 乙二醛 气相色谱 

分 类 号：TS201.2[轻工技术与工程—食品科学]