毕赤酵母表达β-葡萄糖苷酶中试条件优化  

Optimization of pilot-scale conditions of β-glucosidase fermentation by Pichia pastoris

作　　者：王蕊[1] 王林风[1] 闫德冉[1] 赵子高[1] 刘乐[1] 

机构地区：[1]河南天冠企业集团有限公司车用生物燃料技术国家重点实验室,南阳473000

出　　处：《食品科技》2015年第12期15-19,共5页Food Science and Technology

基　　金：国家高技术研究发展计划项目(2012AA10180402);南阳市重大科技攻关项目

摘　　要：通过单因素摇瓶培养结合50 L发酵罐参数优化,对毕赤酵母基因工程菌生产β-葡萄糖苷酶发酵条件进行研究,确定毕赤酵母β-葡萄糖苷酶工程菌摇瓶最佳产酶条件：增值阶段最适初始p H5.5、接种量10%、装液量100 m L/500 m L,50 L发酵罐中试条件：诱导p H6.0、诱导时间96 h、甲醇浓度1.0%（v/v）、溶氧控制20%~30%,在此条件下,酶活力可达98.85 IU/m L。进一步优化,可为提高木质纤维素生产纤维乙醇过程中酶解得率提供技术支撑。To improve the cellulose saccharity rate,the fermentation conditions of Pichia pastoris producing β–glucosidase were studied.By single factor test and parameter optimization for pilotscale,the pilot-scale process were acquired.The optimal shake flask conditions were that initial p H5.5,inoculums size 10%,the volume of liquid 100 m L/500 m L.The fermentation conditions were initial p H6.0,induction time 96 h,methanol concentration 1.0%（v/v）,DO volume 20%~30%,β-glucosidase activity 98.85 IU/m L.For further optimization,the technique can be provided for improving the hydrolysis yield of lignocellulosic.

关 键 词：Β-葡萄糖苷酶 毕赤酵母 发酵条件 诱导 

分 类 号：TS210.3[轻工技术与工程—粮食、油脂及植物蛋白工程]