盐藻β-胡萝卜素微波提取工艺研究  

Study on microwave- assisted extraction technology of β-carotenoid from Dunaliella salina

作　　者：孙协军[1] 薛晓霞[1] 李秀霞[1] 毕海燕[1] 潘龙飞[1] 刘羽纯 

机构地区：[1]渤海大学食品科学与工程学院,辽宁锦州121013

出　　处：《食品工业科技》2016年第1期252-257,共6页Science and Technology of Food Industry

基　　金：辽宁省食品安全重点实验室开放课题(LNSAKF2011015)

摘　　要：建立了一种从盐藻粉中快速提取β-胡萝卜素的微波辅助提取方法,考察了不同溶剂、液固比、温度、时间和搅拌速率对提取效率的影响,同时比较了最佳提取条件下(微波提取、超声波提取和传统溶剂浸提)盐藻β-胡萝卜素的提取效率。优化后的微波提取工艺条件为:乙酸乙酯为溶剂,微波功率500 W,液固比232 m L/g,提取温度42℃,萃取时间7.0 min,搅拌转速180 r/min,在优化条件下,盐藻β-胡萝卜素的得率为1.03%;与传统的溶剂浸提方法相比,微波提取和超声波提取都有效的提高了盐藻β-胡萝卜素的提取效率,且超声波提取的效率最高。A quickly microwave- assisted extraction method was built for β- carotene extraction from Dunaliella salina,different solvents,ratio of liquid to solid,extracting time,extracting temperature,and stirring speed were investigated.The effect of microwave- assisted extraction method was compared with ultrasonic assisted extraction method,and conventional solvent maceration extraction method under optimal conditions.The optimized condition of microwave- assisted extraction method was that ethyl acetate was used as solvent,ratio of liquid to solid232 m L / g,extracting temperature 42 ℃,extracting time 7.0 min,and stirring speed 180 r / min.β- carotene yield of Dunaliella salina was 1.03% when it was extracted under the optimal conditions. Compared to the conventional solvent maceration extraction method,the β- carotene yield was improved significantly by microwave- assisted extraction method and ultrasonic assisted extraction method,respectively,and the maximum β- carotene extraction efficiency was deserved by ultrasonic assisted extraction method.

关 键 词：盐藻 Β-胡萝卜素 乙酸乙酯 微波提取 

分 类 号：TS219[轻工技术与工程—粮食、油脂及植物蛋白工程]