三斑海马水提物抗氧化活性研究  

Antioxidant activity of aqueous extract from Three- spot hippocampus

作　　者：陈莉萍[1] 申铉日[1] 陈国华[2] 孙菲菲[1] 金美娜[1] 

机构地区：[1]海南大学食品学院,海南海口570228 [2]海南大学海洋学院,海南海口570228

出　　处：《食品工业科技》2016年第3期114-118,共5页Science and Technology of Food Industry

基　　金：十二五国家科技支撑计划课题(2013BAB01B04)

摘　　要：本文探讨三斑海马小分子水溶性提取物的抗氧化能力。以三斑海马干燥体粗粉为原料,经95%乙醇回流提取,系统溶剂提取获得不同极性提取物,并对水提物进一步分离及抗氧化活性评价。经Sephadex G-10凝胶过滤层析分离得到6个组分。对组分清除DPPH自由基,超氧阴离子自由基和羟基自由基能力,总还原力等抗氧化能力以及总氨基酸和总酚含量进行测定和评价。实验结果表明,在分离获得的6个组分中FrⅣ的DPPH自由基清除活性最高,其IC50=0.25 mg/m L。FrⅣ的总还原力较高,羟基自由基清除活性及铁离子螯合能力最强。表明三斑海马小分子水溶性提取物的抗氧化活性与总酚、总氨基酸、小分子肽的含量和类型密切相关。The antioxidant capacity of aqueous soluble extract with small molecules of Three- spot hippocampus was investigated.Three- spot seahorse was taken as raw material,extracts were obtained via reflux extraction using95% ethanol and systematic solvent extraction. Aqueous extract was selected for further analysis amongst the resulted various polar extracts. Six fractions were obtained by eluting through Sephadex G- 10 gel filtration chromatography.Anti- oxidative activities of DPPH free radical scavenging,and hydroxyl radical scavenging,total reducing power and ferrous ion chelating ability were determined and evaluated at multiple concentrations and total polyphenols and total amino acids of varying fractions. The results indicated： by contrast,DPPH free radical clearance of Fr Ⅳ was the highest with the of IC_（50） of 0.25 mg / m L. Total reducing power FrⅣ was potent,Fr Ⅳexhibited the highest capacity for scavenging hydroxyl radical and ferrous ion chelating ability. Collectively,the antioxidant activity of aqueous soluble extract with small molecules of Three- spot hippocampus seemed to have correlation with the distinct contents and types of phenolic compounds,amino acids,small molecular peptides.

关 键 词：三斑海马 水提物 抗氧化活性 

分 类 号：TS201.2[轻工技术与工程—食品科学]