解淀粉芽孢杆菌Z16的产酶条件优化及应用  

Culture optimizations and application for Bacillus amyloliquefaciens Z16 to produce neutral protease

作　　者：曾诚[1] 马毛毛 肖彦骏 曾哲灵[3,4] 余平[4] 钟卫民[5] 

机构地区：[1]南昌大学第一临床医学院,江西南昌330031 [2]南昌大学生命科学学院,江西南昌330031 [3]南昌大学食品科学与技术国家重点实验室,江西南昌330047 [4]南昌大学资源环境与化工学院,江西南昌330031 [5]抚州职业技术学院,江西抚州344106

出　　处：《食品工业科技》2016年第3期196-200,205,共6页Science and Technology of Food Industry

基　　金：国家国际科技合作专项项目(2011DFA32770);江西省国际科技合作项目(20112BDH80004;20123BDH80011);南昌市对外科技合作项目(211-DWHZ-SWYY-001);江西省科技支持计划重大项目(20143ACG70015);江西省高等学校科技落地计划项目(KJLD12012);江西省教育厅产学研合作项目(GJJ11003);江西省科技支撑计划项目(GJJ11003)

摘　　要：本文采用单因素实验和正交实验,对解淀粉芽孢杆菌Z16的产酶条件及水酶法提取樟树仁油工艺条件进行优化,并与枯草芽孢杆菌AS1.398的水酶法提取樟树籽仁油工艺进行比较。结果表明,解淀粉芽孢杆菌Z16的最佳发酵培养基为:玉米粉4.5%,麸皮2.5%,豆粕4.0%,Ca Cl_20.2%,KH_2PO_40.03%,Na_2HPO_4·12H_2O 0.4%,p H7.5,121℃灭菌20 min;解淀粉芽孢杆菌Z16的最佳发酵条件为:接种量2%,37℃、220 r/min,摇瓶培养44 h。在最佳产酶工艺条件下,解淀粉芽孢杆菌Z16所产中性蛋白酶活力为6984.3 U/m L,比产酶条件优化前提高了54.0%。解淀粉芽孢杆菌Z16水酶法提取樟树籽仁油的最适酶解时间为4 h、最适加酶量为20%(v/v),相应的樟树籽仁油得率为91.1%、樟树籽仁油的酸价升高值只有0.3 mg KOH/m L。解淀粉芽孢杆菌Z16的水酶法提取樟树籽仁油的效果显著优于枯草芽孢杆菌AS1.398(常用的中性蛋白酶生产菌)的效果。The enzyme- producing conditions of Bacillus amyloliquefaciens Z16 and the technological conditions of aqueous enzymatic extraction of camphor tree seed kernel oil were optimized by using single- factor experiment and orthogonal experiment,meanwhile,compared with the Bacillus subtilis AS1.398.The best fermentation medium composition of Bacillus amyloliquefaciens Z16 was corn meal 4.5%,wheat bran 2.5%,soya bean meal 4.0%,Ca Cl_20.2%,KH_2PO_4 0.03%,Na_2HPO_4· 12H_2 O 0.4%,p H7.5,and the best fermentation conditions of Bacillus amyloliquefaciens Z16 were inoculation amount 2%,temperature 37 ℃,rate 220 r / min,shake flask culture 44 h.Under the best enzyme- producing technological conditions,the activity of neutral protease produced by Bacillus amyloliquefaciens Z16 was 6984.3 U / m L,which was raised by 54.0% after the optimization.The optimum conditions of of Bacillus amyloliquefaciens Z16 aqueous enzymatic extraction of camphor tree seed kernel oil were enzyme adding proportion 20%（ v / v）,enzymolysis time 4 h. The corresponding camphor tree seed kernel oil yield was91.1%,and its acid value only rose by 0.3 mg KOH / m L. The effect of Bacillus amyloliquefaciens Z16 on aqueous enzymatic extraction of camphor tree seed kernel oil was obviously superior to Bacillus subtilis AS1.398 commonlyused to produce neutral protease.

关 键 词：解淀粉芽孢杆菌 中性蛋白酶 樟树籽仁油 水酶法 

分 类 号：TS201.3[轻工技术与工程—食品科学]