板栗壳鞣质提取及其对DPPH自由基清除活性的研究  

Study on tannins extraction process from Chestnut shell and scavenging effects of DPPH free radical

作　　者：郑佳欣 李怡婧 汪晨阳 章嘉男 史玲玲[1] 王建中[1] 

机构地区：[1]北京林业大学生物科学与技术学院,北京100083

出　　处：《食品工业科技》2016年第3期211-215,共5页Science and Technology of Food Industry

基　　金：国家林业公益性行业科研专项(201204401);北京市级项目:北京林业大学"北京市大学生科学研究与创业行动计划"项目(S201410022042)

摘　　要：本研究以板栗加工的废弃物板栗壳为原材料,以期得到板栗壳中鞣质的最佳提取工艺,并对其抗氧化活性进行考察。通过分析比较筛选出了板栗壳鞣质提取最佳溶剂为乙醇,较优提取方法为回流法,并在此基础上实施了乙醇浓度、料液比和提取时间三个单因素实验和以此为依据的正交实验。结果表明,回流法提取板栗壳鞣质的最佳工艺条件为:25%乙醇为提取溶剂,提取时间为2 h,料液比为1∶10.5(g∶m L),在该条件下,板栗壳鞣质的提取量为14.617 mg/g;DPPH实验结果表明板栗壳鞣质粗提物具有较强的清除DPPH自由基的能力,可以作为一种天然抗氧化剂予以开发。The aim of the study was to obtain the optimum extraction method of tannins and study its scavenging effects of DPPH free radical from chestnut shell,which is a kind of waste in chestnut production.It was determined that the optimum extraction method was refluxing method and the optimum extraction solvent was ethanol by screening experiments. On the basis,single factor experiments and orthogonal experiments were carried out. The effects of different volume fractions of ethanol,extraction time and solid- liquid ratio were explored. And the optimum extraction conditions were that 25% aqueous ethanol solution as the best extraction solvent,the solid /liquid ratio of 1 to 10.5 and extraction time 2 h. Under these conditions,the extraction ratio for the chestnut shell tannin reached 14.617 mg / g.The antioxidant activity of tannin crude extracts was investigated by DPPH method,and the results showed that tannin crude extracts from chestnut shell had strong antioxidant effect and it could be explored as a kind of natural antioxidants.

关 键 词：板栗壳 鞣质 回流法 正交实验法 抗氧化活性 

分 类 号：TS201.1[轻工技术与工程—食品科学]