离子色谱法测定常见海藻及琼脂中的硫酸根含量  

Analysis of Sulfate Contents in Common Seaweeds and Agars by Ion Chromatography

作　　者：王萍萍[1] 倪辉[1,2,3] 姜泽东[1,2,3] 杨远帆[1,2,3] 朱艳冰[1,2,3] 肖安风[1,2,3] 李利君[1,2,3] 杜希萍[1,2,3] 陈峰[1,4] 

机构地区：[1]集美大学食品与生物工程学院,福建厦门361021 [2]福建省食品微生物与酶工程重点实验室,福建厦门361021 [3]厦门市食品与生物工程技术研究中心,福建厦门361021 [4]美国克莱姆森大学食品科学营养与包装科学系,美国南卡罗琳娜州29634

出　　处：《集美大学学报（自然科学版）》2016年第1期29-34,共6页Journal of Jimei University：Natural Science

基　　金：国家自然科学基金资助项目(31501441);海洋公益性行业科研专项项目(201505033-2);厦门市海洋与渔业局项目(14GZP010NF10)

摘　　要：为测定常见海藻及琼脂中的硫酸根含量,建立了测定硫酸根含量的离子色谱内标法,对6种琼脂和5种海藻中的硫酸根含量进行测定.结果表明：选择KNO3作为内标物,硫酸根的质量浓度在10~45 mg/L范围内线性关系良好（R2〉0.999）,加标回收率为93.1%~106.4%.该方法测得的琼脂中硫酸根质量分数在0.25%~1.21%之间,海带、紫菜、麒麟菜、红毛菜和江蓠中硫酸根质量分数分别为2.58%、3.86%、10.62%、2.42%和1.01%,其中麒麟菜的显著高于其他4种海藻的（P〈0.05）.In order to investigate the sulfate contents in common seaweeds and agars, five seaweeds and six agars were analyzed by using an internal standard with ion chromatography （IC）, which was then used to calculate the sulfate content. The result showed that the chosen KNO3 was more suitable as the internal standard for quantify of SO24-. The contents of SO4^2- exhibited well lineafity in a range of 10 -45 mg/L （R2 〉 0. 999） , and the recoveries were 93.1% - 106.4%. By using the above method, sulfate contents exhibited a range from 0. 25% to 1.21% in different agars, besides, the sulfate contents of kelp, nori, echeuma, bangia and gracilaria were 2.58% , 3.86% , 10. 62% , 2.42% and 1.01% , respectively. In addition, the sulfate content of echeuma was significantly higher than that of the others （P 〈 0.05 ） .

关 键 词：离子色谱 琼脂 海藻 内标法 硫酸根 

分 类 号：Q539.9[生物学—生物化学] O657.7[理学—分析化学]