超高效液相色谱——三重四级杆串联质谱仪(UPLC-MS/MS)同时测定白酒中7种有机酸的分析研究  

Ultra Performance Liquid Chromatography-triple Quadrupole Tandem Mass Spectrometry(UPLC-MS/MS) Simultaneously Determine 7 Kinds of Organic Acids in Liquor

作　　者：王银辉[1,2] 沈小梅[1,2] 马雷[1,2] 李安军[1,2] 刘国英[1,2] 汤有宏 

机构地区：[1]安徽古井贡酒股份有限公司,安徽亳州236820 [2]安徽省固态发酵工程技术研究中心,安徽亳州236820

出　　处：《酿酒》2016年第1期43-46,共4页Liquor Making

摘　　要：为检测白酒中微量有机酸（没食子酸、阿魏酸、儿茶素、咖啡酸、木犀草素、绿原酸和对香豆酸）,建立了一种利用超高效液相色谱-三重四级杆串联质谱仪（ESI离子源）的检测方法。样品经挥酒精前处理后,以HSS T3色谱柱为分离柱,0.1%（v/v）乙酸-1mmol/L乙酸铵溶液/乙腈为流动相进行梯度洗脱,经超高效液相色谱（UPLC）分离,采用电喷雾串联四级杆质谱进行多反应监测模式（MRM）进行检测。7种有机酸的线性范围在20~200ng/m L内,拟合所得线性相关系数均大于0.992。在3个加标水平下,得到样品平均回收率为97.1%~111.3%,相对标准偏差（RSD）均控制在5%以内。该方法简单快捷、准确可靠,可用于白酒中7种有机酸的同时检测。For detection of trace organic acids in liquor（gallic acid, ferulic acid, catechin, caffeic acid, mignonette element, chlorogenic acid and the coumaric acid）, this paper established a using ultra high performance liquid chromatography（HPLC）- triple level 4 pole tandem mass spectrometry（ESI ion source） detection method. Sample after wave alcohol before treatment, for HSS T3 chromatographic column separation column, 0.1%（v/v） acetic acid ammonium acetate solution- a tendency for L/acetonitrile as mobile phase, gradient elution by ultra high performance liquid chromatography（UPLC） separation, mass spectrometry using electrospray tandem level 4 rod to carry on the multiple reaction monitoring mode（MRM） for testing. 7 kinds of organic acids in the linear range within 20 to 200 ng/m L, fitting the linear correlation coefficients were greater than 0.992. Get the sample in three level of the standard addition, the average recovery was 81.25% ~81.25%, the relative standard deviation（RSD） are controlled within 5%. The method is simple and quick, accurate and reliable and can be used for 7 kinds of organic acids in liquor detection at the same time.

关 键 词：没食子酸 阿魏酸 儿茶素 咖啡酸 木犀草素 绿原酸 对香豆酸 液质联用仪 

分 类 号：TS262.3[轻工技术与工程—发酵工程] TS207.3[轻工技术与工程—食品科学与工程]