黄皮疣柄牛肝菌多酚提取及对Caco-2结肠癌细胞抑制作用研究  

The polyphenol extraction of Leccinellum crocipodium and their inhibitory effect on proliferation of Caco-2 cells

作　　者：刘雨阳[1] 侯玉艳[1] 吴素蕊[2] 赵天瑞[1] 樊建[1] 

机构地区：[1]昆明理工大学云南省食品安全研究院,云南昆明650500 [2]中华全国供销合作总社昆明食用菌研究所,云南昆明650221

出　　处：《食品工业科技》2016年第4期278-282,共5页Science and Technology of Food Industry

基　　金：国家科技支撑计划课题(2013BAD16B01)

摘　　要：以黄皮疣柄牛肝菌为原料,乙醇为提取溶剂,采用Folin-Ciocalteu法测定多酚含量,研究了乙醇浓度、提取温度、料液比和提取时间对多酚得率的影响。在单因素实验的基础之上,通过正交实验优化提取工艺参数,并测定了所得多酚组成及其对Caco-2结肠癌细胞的抑制作用。结果表明,最佳提取工艺条件为:温度为80℃,乙醇浓度为50%,料液比为1∶25 g/m L,提取1 h。在此提取工艺条件下,最佳提取次数为2次时,黄皮疣柄牛肝菌多酚的得率为5.46%±0.04%;在18种酚类物质中,在黄皮疣柄牛肝菌中检出5-磺基水杨酸、儿茶素和肉桂酸等8种多酚类物质,其中5-磺基水杨酸含量最高为779.00 mg/100 g;黄皮疣柄牛肝菌多酚对Caco-2结肠癌细胞产生一定程度的抑制作用,当其浓度为175μg/m L时,抑制率达55.07%。The polyphenols were extracted from the Leccinellum crocipodium by ethanol as extraction solvent extraction and Folin-Ciocalteu method. The effect of ethanol concentration,temperature,solid-liquid ratio and time on the extraction efficiency of polyphenols were identified. Based on single factor test,an orthogonal design was applied to optimize extraction process. The polyphenol composition and inhibitory effect on Caco-2 cells of Leccinellum crocipodium were investigated in the present work. The results showed that the optimum conditions for extraction of polyphenols were temperature of 80 ℃,ethanol concentration of 50%,solid-liquid ratio of 1∶25,extracted 1 h,two times. Under this condition,polyphenol extraction rate was 5.46% ±0.04%.Among 18 kinds of polyphenols,the polyphenols of this wild edible fungi were mainly composed of 5-sulfosalicylic acid,catechin,cinnamon and other five kinds of polyphenols,and the content of 5-sulfosalicylic acid up to779.00 mg/100 g. The inhibitory effects on colon carcinoma cells showed that the cell inhibition rate was up to55.07%,when the polyphenols concentration was 175 μg/m L.

关 键 词：黄皮疣柄牛肝菌 多酚 Caco-2结肠癌细胞 抗癌 

分 类 号：TS201.3[轻工技术与工程—食品科学]