紫外-等离子体复合诱变红曲霉产胞外多糖  

Research on screening and breeding of Monascus on the production of extracellular polysaccharides by UV-ARTP composite mutagenesis

作　　者：蒋汶 张庆庆[1] 汤文晶[1] 程亚运 

机构地区：[1]安徽工程大学生物与化学工程学院,安徽芜湖241000

出　　处：《食品与发酵工业》2016年第1期64-69,共6页Food and Fermentation Industries

基　　金：安徽高校省级自然科学研究重点项目(KJ2009A034);芜湖市重点科技项目(芜科计字[2009]190号)

摘　　要：以红曲霉ZL307为出发菌株,采用紫外结合常压室温等离子体的复合诱变方法对其进行诱变。紫外诱变条件为:照射距离15 cm,功率15 W,时间90 s。等离子体诱变条件为:照射距离3 mm,注入气体氦气,气流量10 L/min,功率200 W,时间180 s。筛选得到具有良好遗传稳定性的菌株ZJ307-3,与原始菌株相比,发酵周期没有发生明显的改变,7 d时多糖产量达到550.07 mg/L,提高61.18%。复合诱变菌株多糖产量较单紫外诱变菌株提高27.58%,较单等离子体诱变菌株提高12.55%。The parent strain Monascus ZL307 was mutated by UV-ARTP composite mutagenesis. The UV mutation conditions were as follows： the distance was 15 cm and the time of irradiation was 90 s with a 15 W ultraviolet lamp. The ARTP mutation conditions were as follows： the distance was 3 mm,the injected gas was helium with a rate of 10 L / min,the irradiation power was 200 W and the time was 180 s. ZJ307-3,a higher production of polysaccharide strain,was obtained. The genetic stability of this strain was well. There were no significant changes during its fermentation period. Its polysaccharide production reached 550. 07 mg / L on day 7. Its polysaccharide production was61. 18% higher than that of parent strain. The polysaccharide production of the composite mutant strain was 27. 58%higher than that of the UV mutant strain and 12. 55% higher than that of the ARTP mutant strain.

关 键 词：红曲霉 胞外多糖 紫外诱变 等离子体诱变 

分 类 号：TS201.3[轻工技术与工程—食品科学] TQ929[轻工技术与工程—食品科学与工程]