模式类黑精及其分级产物清除自由基能力、抗氧化性及还原力分析  

Analysis of free radical-scavenging and reducing ability of melanoidins and its fractions

作　　者：王忠合[1] 王军[1] 胡慧娟[1] 

机构地区：[1]韩山师范学院生命科学与食品科技学院,广东潮州521041

出　　处：《食品与发酵工业》2016年第1期92-96,共5页Food and Fermentation Industries

基　　金：广东省自然科学基金项目(S2013040015478);广东省高校优秀青年创新人才培养计划项目(2013LYM0056);韩山师范学院青年计划项目(LQ201202);韩山师范学院博士启动项目(QD20130516);韩山师范学院一般项目(LY201306);潮州市科技计划项目(2013X05&2013X06&2014N02)资助

摘　　要：以甘氨酸和葡萄糖为底物制备模式类黑精,采用膜法制备分级产物,分析模式类黑精及其分级产物的自由基清除能力、抗氧化性及还原力。结果表明:水溶性类黑精及其分级产物具有较好的清除羟自由基、超氧阴离子和DPPH自由基的能力;鸡卵黄脂质过氧化模型分析表明模式类黑精及其分级产物的抗脂质过氧化性差异较大,模式类黑精和高分子质量的类黑精抗氧化效果较理想;采用铁氰化钾法和二氯酚定酚法测定模式类黑精及分级产物的还原力表明,高分子质量的类黑精还原力最大。In this paper,standard melanoidins derived from glycine-glucose model system were prepared using Maillard reaction and ultrafiltration methods,and radical-scavenging ability,antioxidant ability and reducing capacity of standard melanoidins and its fractions were also investigated. Results showed that water-soluble melanoidins and fractions possessed stronger radical-scavenging ability,including hydroxyl radical,superoxide anion and DPPH radical. However,only water-soluble melanoidins showed better antioxidant properties in yolk lipid peroxidation model.Finally,high molecular weight fraction（ HMW） has the best reducing capacity by potassium ferricyanide and 2,6-dichloroindophenol evaluation methods.

关 键 词：类黑精 清除自由基 抗氧化 还原力 

分 类 号：TS201.2[轻工技术与工程—食品科学]