啤酒麦芽中极限糊精酶的分离纯化  

Purification of Limit Dextrinase from Malted Barley

作　　者：付燕秋[1,2] 管斌[1,2] 孔青[1] 赵旭乐 余俊红 董建军 

机构地区：[1]中国海洋大学食品科学与工程学院,山东青岛266003 [2]啤酒生物发酵工程国家重点实验室,山东青岛266061

出　　处：《中国食品学报》2015年第12期122-127,共6页Journal of Chinese Institute Of Food Science and Technology

基　　金：山东省自然科学基金项目(ZR2010CM050);青岛市公共领域科技支撑计划项目(11-2-3-63-nsh)

摘　　要：以发芽6 d的澳麦麦芽为原料,通过醋酸钠缓冲液萃取,硫酸铵分部盐析,Sephadex G-25凝胶过滤柱层析,DEAE Sepharose FF离子交换柱层析及Sephadex G-100凝胶柱层析,获得极限糊精酶,其比活力由3.40m U/mg提高到558.26 m U/mg,纯化倍数和回收率分别为164.2倍和16.5%。该酶经SDS-PAGE检测分子质量约为101.9 ku,IEF检测等电点p I约为4.66,并且电泳图谱均显示单一条带,说明该纯化酶达到电泳纯。The crude enzyme solution of limit dextrinase was extracted by sodium acetate buffer from six days green malt of Australia. The malt limit dextrinase was purified with ammonium sulfate precipitation, Sephadex G-25 gel filtration chromatography, DEAE Sepharose FF anion exchange chromatography and Sephadex G-100 gel filtration chromatography from the extracted enzyme solution. The enzyme was purified to 164.2-fold with 16.5% recovery, and the specific activity increased from 3.40 mU/mg to 558.26 mU/mg. SDS-PAGE and IEF analysis revealed a single protein band corresponding to a molecular mass of 101.9 ku and a isoelectric point of 4.66, and the single protein band also showed that the purified enzyme has reached electrophoretically pure.

关 键 词：啤酒麦芽 极限糊精酶 分离纯化 

分 类 号：TS262.5[轻工技术与工程—发酵工程]