山杏花总黄酮抗氧化活性及其对酪氨酸酶抑制作用的研究  

Antioxidant activity and inhibitory activity on Tyrosinase of total flavonoids from Siberian Apricot flowers

作　　者：宁亚萍[1] 董施彬[1] 李建霞[1] 杨喆[1] 张乔会[1] 董洁琼[1] 王建中[1] 

机构地区：[1]林业食品加工与安全北京市重点实验室生物科学与技术学院北京林业大学,北京100083

出　　处：《食品工业科技》2016年第5期104-108,共5页Science and Technology of Food Industry

基　　金：林业公益性行业科研专项(201004081)

摘　　要：利用闪式提取法提取山杏花总黄酮,并以X-5大孔树脂进行分离。通过测定分离前后山杏花总黄酮对DPPH自由基、羟基自由基的清除能力,总还原能力和总抗氧化能力,评价了其抗氧化活性;通过测定酪氨酸酶催化L-多巴氧化速率,以V_C和β-熊果苷为阳性对照,研究了分离前后山杏花总黄酮对体外酪氨酸酶活性的抑制作用。结果表明,分离前后山杏花总黄酮均具有一定的抗氧化活性,且分离后抗氧化活性得到明显提高;分离前后山杏花总黄酮对酪氨酸酶的抑制能力均强于β-熊果苷弱于V_C,其IC_(50)值分别为2.24、3.08 mg/m L。综上所述,分离前后山杏花总黄酮均具有一定的抗氧化活性,且能有效抑制酪氨酸酶的活性。Total flavonoids from Siberian apricot flowers were extracted with the method of flash extraction and purified with X-5 macroporous resins.The antioxidant activity of separated and unseparated total flavonoids from Siberian apricot flowers were investigated by measuring scavenging effect on DPPH· and OH·, total reduction capacity,total antioxidant capacity and the inhibitory effect on tyrosinase activity was studied by measuring the rate of tyrosinase catalyzed L-DOPA oxidation taking Vc and /3-arbutin as positive controls.The results showed that separated and unseparated total flavonoids from Siberian apricot flowers had a certain antioxidant activity.After separation,the antioxidant activity was improved obviously, the inhibitory effect on tyrosinase of separated and unseparated total flavonoids from Siberian apricot flowers was better than β-arbutin but worse than Vc.Their half maximal inhibitory concentration （ IC50 ） were 2.24, 3.08 mg/mL, respectively. In conclusion, separated and unseparated total flavonoids from Siberian apricot flowers had a certain antioxidant activity and can effectively inhibit tyrosinase activity.

关 键 词：山杏花总黄酮 抗氧化活性 酪氨酸酶 抑制作用 

分 类 号：TS201.1[轻工技术与工程—食品科学]