番茄红素提纯工艺条件的研究  

Research of purification technology of lycopene

作　　者：杨阳 刘蕊 王英明 张玉丹 李岩 陈宝瑾[3] 任航[4] 

机构地区：[1]新疆红帆生物科技有限公司,新疆库尔勒841100 [2]中国专利技术开发公司,北京100088 [3]上海市浦东新区浦南医院,上海200125 [4]哈尔滨市食品药品检验检测中心,黑龙江哈尔滨150025

出　　处：《中国酿造》2016年第3期111-113,共3页China Brewing

摘　　要：以番茄红素油树脂为原料,利用皂化法和重结晶法作为主要提纯工艺,纯化得出高含量的番茄红素晶体。通过单因素及正交试验,确定番茄红素提纯工艺的最佳提取条件为番茄红素油树脂∶丙三醇∶氢氧化钾∶无水乙醇比例4∶3∶1∶1(V/V),皂化温度70℃,皂化时间2.5 h,重结晶时间48 h,在此条件下番茄红素纯度达到61.64%。Using the lycopene oleoresin as raw material, the lycopene crystal with high content was obtained by saponification and recrystallization method which were the main purification technology. By single factor and orthogonal experiments, the optimum extraction conditions of lycopene were determined as lycopene oleoresin： glycerol： potassium hydroxide： absolute ethyl alcohol ratio 4：3：1：1 （V/V）, saponification temperature 70 ℃, saponification time 2.5 h and recrystallization time 48 h. Under the conditions, the purity of lycopene was up to 61.64%.

关 键 词：番茄红素油树脂 皂化法 重结晶法 提纯工艺 

分 类 号：TS264.2[轻工技术与工程—发酵工程]