羊胎盘免疫活性肽的制备及其活性  

The Preparation and Bioactivity of Goat Placenta Immune Activity Peptides

作　　者：侯银臣 吴丽[2] 刘旺旺[3] 程永霞[3] 周洁静[3] 杨公明[3] 

机构地区：[1]河南省商业科学研究所有限责任公司,郑州450000 [2]河南牧业经济学院,郑州450011 [3]华南农业大学食品学院,广州510642

出　　处：《中国食品学报》2016年第1期123-129,共7页Journal of Chinese Institute Of Food Science and Technology

摘　　要：利用枯草芽孢杆菌在发酵过程中产蛋白酶的特点,对羊胎盘下脚料发酵制备羊胎盘免疫活性肽。以产物对小鼠脾脏淋巴细胞的刺激指数为指标,通过响应面法对发酵过程中的重要参数进行优化,考察了不同质量浓度下的发酵液单独和与LPS、ConA共同作用对小鼠脾淋巴细胞的刺激作用。采用超滤技术对发酵产物进行分离,研究不同分子质量范围的组分的免疫与抗氧化活性。研究表明：枯草芽孢杆菌发酵羊胎盘下脚料制备免疫活性肽的最佳发酵条件是：料液pH 5.42、碳源含量1.73%、发酵时间43.45 h,在该条件下的发酵液对小鼠脾淋巴细胞的刺激效果显著,当发酵液蛋白质量浓度为100μg/mL时,小鼠脾淋巴细胞的刺激指数达到23.26%,与LPS、ConA共同作用免疫效果显著,最适质量浓度分别为100,50μg/mL。超滤试验表明,不同分子质量组分的免疫活性和抗氧化活性不同,3-10 ku组分的免疫活性最高;3-10 ku和〈3 ku组分的抗氧化活性最高。Bacillus subtilis is one of main bacterium of producing industrial enzymes. The goat placenta immune active peptides were prepared through fermentation with Bacillus subtilis. The objective of the present work was to investigate the effects of fermentation parameters（content of carbon sources, pH and time） on spleen lymphocyte proliferation for the highest immune activity of the fermentation broth using response surface methodology（RSM）. The optimal fermentation methods were obtained under the conditions of 1.73% of carbon sources, initial pH 5.42, and 43.45 h of fermentation time. Under the optimized fermentation conditions, fermentation broth in a certain concentration range（6.25-200 μg/mL） showed significant effect of proliferation of mouse spleen lymphocytes. In stimulation alone, a dose of 100 μg/mL stimulated mouse spleen lymphocytes most significantly, while in the synergistic effect, a dose of 50 μg/mL played the most significant role with ConA, a dose of 100 μg/mL played the most significant role with LPS. Ultrafiltration technique was performed to separate fermentation broth with different molecular weight. It was found that peptides in the range of3-10 ku mainly accounted for the immune activity and peptides in the range of 3-10 ku and 〈3 ku mainly accounted for the antioxidant activity.

关 键 词：枯草芽孢杆菌 羊胎盘 免疫活性 抗氧化活性 肽 

分 类 号：TQ929[轻工技术与工程—发酵工程]