食品中甲醛残留量高通量检测方法研究  

The Study of High-throughput Detection Method for Formaldehyde Residues in Food

作　　者：潘红艳[1] 郭启雷[1] 史海良[1] 杨红梅[1] 

机构地区：[1]国家食品质量安全监督检验中心,北京100094

出　　处：《食品研究与开发》2016年第3期142-145,共4页Food Research and Development

基　　金：公益性行业科研专项项目(2012104003-2)

摘　　要：建立食品中甲醛残留量的高通量快速检测方法。样品中的甲醛在酸性条件下与2,4-二硝基苯肼发生加成反应,经高效液相色谱紫外检测器检测,流动相乙腈∶水(6∶4,体积比),流速1.0 m L/min,检测波长355 nm。该方法在0.05μg/m L^5.0μg/m L质量浓度范围内线性关系良好(R2=0.999 9),检出限为0.5 mg/kg,加标回收率为80.2%~107.7%。该方法操作简便、快速、实用性广,可为面粉、粉条、虾皮、竹笋、腐竹、鱼类(鱿鱼、鲫鱼等)、血豆腐等多种食品基质中甲醛残留量的检测提供参考。A rapid detection method was developed. The samples were addition with 2,4-dinitrophenylhydrazine under acidic conditions, detected by HPLC UV detector. The mobile phase was acetonitrile ∶ water（6 ∶ 4, m L/m L）, at a pump flow rate of 1.0 m L/min, detection wavelength of 355 nm. Under the optimal conditions, the linear range of formaldehyde was 0.05 μg/kg-5.0 μg/kg with correlation coefficient higher than 0.999 9, the limit of quantification was 0.5 mg/kg, the mean recoveries were 80.2 %-107.7 %. This method was simple, rapid and suitable for most food matrices such as flour, vermicelli, shrimp, bamboo shoots, yuba, fish（Carp, Squid etc）, blood tofu etc.

关 键 词：甲醛 高效液相色谱 食品 

分 类 号：TS207.5[轻工技术与工程—食品科学]