鱼皮低聚肽氨基酸组成及抗氧化活性研究  

Study on Amino Acid Composition and Antioxidative Activity of Fish Skin Oligopeptides

作　　者：王宁丽[1,2] 徐月敏[1,2] 刘毅[2,3] 刘永峰[2,3] 张振兴[2,3] 邸多隆[2,3] 刘晔玮[1] 

机构地区：[1]兰州大学公共卫生学院营养与食品卫生学研究所,甘肃兰州730000 [2]中国科学院兰州化学物理研究所中科院西北特色植物资源化学重点实验室和甘肃省天然药物重点实验室,甘肃兰州730000 [3]青岛市资源化学与新材料研究中心,山东青岛266100

出　　处：《食品研究与开发》2016年第2期31-35,共5页Food Research and Development

基　　金：青岛市民生科技计划项目(14-2-3-51-nsh);"西部之光"人才培养计划

摘　　要：以还原型谷胱甘肽和抗坏血酸为对照,采用DPPH·法和邻二氮菲-Fe^(2+)氧化法分别测定鱼皮低聚肽清除DPPH·和·OH活性能力;并利用GB/T5009.124-2003《食品中氨基酸的测定》方法测定鱼皮低聚肽的氨基酸组成,进行营养价值评价及抗氧化能力与氨基酸组成关系的初步分析。结果表明,在优选的反应体系中,鱼皮低聚肽、谷胱甘肽和抗坏血酸对·OH的IC_(50)值分别为2.482、0.357、0.100mg/mL,对DPPH·的IC_(50)值分别为0.635、0.030、0.003 mg/mL,鱼皮低聚肽必需氨基酸含量为24.43%,EAA/TAA值为24.19%,EAA/NEAA值为32%,CS均大于100,EAAI为352,含硫氨基酸含量为3.8%,除蛋氨酸外的疏水性氨基酸占氨基酸总量的26.7%,具有抗氧化活性的氨基酸总量为30.5%,与鱼皮低聚肽的抗氧化活性一致。Under the optimum conditions using glutathione and ascorbic acid as the controls, DPPH· and phenanthroline-Fe2＋oxidation assays were applied to evaluate the capacities for scavenging free radicals of fish skin oligopeptides. Determination of amino acid composition of fish skin oligopeptides put to use the GB/T5009.124-2003（Determination of amino acids in foods） method. The relationship between antioxidative activity and amino acid composition of fish skin oligopeptides was analyzed, and the nutritional values of those were evaluated. The results showed that the values of half-inhibitory concentration（IC50） of fish skin oligopeptides,glutathione and ascorbic acid for ·OH were 2.482, 0.357, 0.100 mg/m L, respectively, and those for DPPH·were 0.635, 0.030 and 0.003 mg/m L, respectively. Essential amino acids contents of fish skin oligopeptides were 24.43 %, EAA/TAA was 24.19 %, EAA/NEAA was 32 %, CS was greater than 100, and EAAI was 352.Sulphur amino acids contents were 3.8 %, hydrophobic amino acids except methionine accounted for 26.7 % of total amino acids, so the total antioxidative activity amino acids were 30.5 % which was consistent with the antioxidative activity of fish skin oligopetides.

关 键 词：鱼皮低聚肽 抗氧化活性 含硫氨基酸 疏水性氨基酸 

分 类 号：TS254.9[轻工技术与工程—水产品加工及贮藏工程]