响应面法优化深黄被孢霉产油突变株的发酵培养基配方  

Optimization of fermentation medium formula for the production of oil from Mortierella isabellina mutant using response surface methodology

作　　者：张敏[1] 关随霞[1] 张玉先[1] 韩四海[1] 

机构地区：[1]河南科技大学食品与生物工程学院,河南洛阳471023

出　　处：《中国油脂》2016年第3期66-69,共4页China Oils and Fats

基　　金：国家自然科学基金(31471658)

摘　　要：为了优化深黄被孢霉突变株的发酵培养基,在单因素实验基础上,采用响应面法(RSM)对深黄被孢霉产油突变株发酵培养基配方进行优化。结果表明,深黄被孢霉产油突变株最佳发酵培养基配方为:葡萄糖90.29 g/L,酵母膏4.14 g/L,Mg SO40.96 g/L,KH2PO41.99 g/L,p H 6.0。在最佳条件下油脂产量为12.01 g/L,比优化前(6.22 g/L)提高了93.09%。The fermentation medium formula for oil production by mutant strains Mortierella isabellina was optimized by response surface methodology on the basis of single factor experiment. The optimal fermentation medium formula for oil production by mutant strains Mortierella isabellina was obtained as follows：glucose 90. 29 g / L,yeast extract 4. 14 g / L,Mg SO40. 96 g / L,KH2PO41. 99 g / L,p H 6. 0. Under the optimal conditions,the oil yield reached 12. 01 g / L,increasing by 93. 09% than original yield（ 6. 22 g / L）.

关 键 词：深黄被孢霉 突变株 微生物油脂 培养基 响应面法 

分 类 号：TQ929[轻工技术与工程—发酵工程]