挤压-酶解联用抗性淀粉工艺的优化  

Optimization of Preparation Process of Resistant Starch by Extrusion and Enzymatic Hydrolysis

作　　者：肖志刚[1] 任海斌[1] 

机构地区：[1]沈阳师范大学粮食学院,沈阳110034

出　　处：《粮食加工》2016年第2期27-33,共7页Grain Processing

基　　金："十二五"农村领域国家科技支撑计划课题(编号:2012BAD34B02);黑龙江省教育厅科学技术研究重点项目(编号:12511z006);哈尔滨市优秀学科带头人基金(编号:2012RFXXN107);辽宁省级公关项目(编号:2014205007);沈阳市农业科技攻关专项(编号:F14-104-3-00)

摘　　要：为制备高抗性淀粉含量玉米淀粉,并拓展抗性淀粉制备方法,以玉米淀粉为原料,采用双螺杆挤压机挤压技术与普鲁兰酶酶解技术联用的方法,研究了物料含水率、螺杆转速、机筒温度和喂料速度对抗性淀粉含量的影响,并通过SPSS线性回归分析确定了最优工艺条件为:物料含水率20%、螺杆转速300 r/min、机筒温度145℃、喂料速度37.5 kg/h,在此条件下抗性淀粉含量达(24.72±0.87)%。通过样品中直链淀粉含量、抗性淀粉含量、扫描电镜图和X-射线衍射图对比,说明通过挤压-酶解联用技术制备的淀粉直链淀粉含量和抗性淀粉含量增加,淀粉颗粒形成大小不匀的多孔疏松结构,淀粉晶体类型由A型转变为B+V型。In order to prepare a high content maize starch, and expand the resistant starch preparation method,in this study, resistant starch was prepared by extrusion- pullulanase hydrolysis using the corn starch as raw material, the influence of the material moisture content, screw speed, barrel temperature and feed rate on the resistant starch content was studied. The optimal process conditions were determined through the SPSS linear regression analysis： 20% moisture content of material, screw speed 300 r/min, barrel temperature 145 ℃, feeding speed37.5 kg/h. Under this condition, the resistant starch content reached at 24.72 ＋ 0.87%. The contrast of the amylose content, resistant starch content, scanning electron microscope（SEM） and X-ray diffraction before and after extursion has shown that amylose content and resistant starch content increased, starch granules formed the uneven size of porous structure and starch crystal type changed from type A intothe B＋V type.

关 键 词：挤压膨化 普鲁兰酶 抗性淀粉 线性回归 淀粉晶体类型 

分 类 号：TS234[轻工技术与工程—粮食、油脂及植物蛋白工程]