牡丹叶黄酮的酶法提取条件优化及其对亚硝酸盐的清除作用  

Optimization of Enzymatic Extraction and Nitrite Scavenging Capacity of Flavonoids from Peony Leaves

作　　者：李佩艳[1] 韩四海[1] 罗登林[1] 赵胜娟[1] 刘建学[1] 尹飞[2] 

机构地区：[1]河南科技大学食品与生物工程学院,河南洛阳471023 [2]河南科技大学农学院,河南洛阳471023

出　　处：《食品科学》2016年第6期77-81,共5页Food Science

基　　金：河南科技大学博士科研启动基金项目(4009-13480053);河南科技大学青年科学基金项目(2015QN035);河南科技大学教育教学改革项目(2015YB-068);河南科技大学创新团队项目(2015XTD007)

摘　　要：以牡丹叶为原料,采用酶法提取牡丹叶黄酮,以纤维素酶用量、p H值、酶解温度、酶解时间为单因素,以牡丹叶黄酮提取率为考察指标,通过正交试验确定酶法提取牡丹叶黄酮的最佳工艺参数为:纤维素酶用量12.5 U/m L、p H 4.5、酶解温度45℃、酶解时间4 h。在此条件下牡丹叶黄酮提取率为2.43%。提取得到的牡丹叶黄酮浸提液可用于亚硝酸盐清除,通过正交试验优化得到牡丹叶黄酮清除亚硝酸盐的最佳反应条件为:反应温度70℃、p H 4.0、牡丹叶黄酮提取液添加量25 m L(10μg亚硝酸钠)、反应时间20 min。在此条件下牡丹叶黄酮对亚硝酸盐清除率可达62.15%。Cellulose-assisted extraction was applied to the extraction of flavanoids from peony leaves. Effects of enzyme dosage, p H, temperature and hydrolysis time on the extraction efficiency of flavanoids were investigated. The optimal hydrolysis conditions obtained by orthogonal array experiments for the extraction of flavonoids were as follows： enzyme dosage, 12.5 U/m L; initial p H, 4.5; temperature, 45 ℃; and hydrolysis time, 4 h. Under these conditions, the extraction yield of flavanoids was 2.43%. Flavanoids from peony leaves were able to scavenge nitrite. The optimal reaction conditions for nitrite scavenging by the flavanoids were found to be 20 min reaction at 70 ℃ and p H 4.0 after addition of 25 m L（10 μg sodium nitrate） of the radical scavenger, resulting in a scavenging rate of 62.15%.

关 键 词：牡丹叶 黄酮 纤维素酶 亚硝酸盐 清除 

分 类 号：TS201.1[轻工技术与工程—食品科学]