HPLC-PDA法测定明日叶不同部位中的4-羟基德里辛和黄色当归醇含量  

Determination of 4-Hydroxyderricin and Xanthoangelol in Different Parts of Angelica keiskei by HPLC-PDA

作　　者：李亚丽[1,2] 罗玉兰[2] 刘建华[3] 吴金鸿[4] 钟耀广[1] 王正武[4] 

机构地区：[1]上海海洋大学食品学院,上海201306 [2]上海市园林科学研究所,上海200232 [3]宜宾学院化学与化工学院,四川宜宾644000 [4]上海交通大学农业与生物学院,上海200240

出　　处：《食品科学》2016年第6期142-145,共4页Food Science

基　　金：上海市科技兴农重点攻关项目(沪农科攻字(2013)第1-5号);国家自然科学基金面上项目(21276154;31171642;31471623);宜宾市科技厅项目(2013ZSF007);中国博士后科学基金项目(2014M561477)

摘　　要：采用高效液相色谱配备光电二极管阵列检测器法测定明日叶根、茎和叶中4-羟基德里辛（4-hydroxyderricin,4-HD）和黄色当归醇（xanthoangelol,XAG）的含量。样品分析选用Halo C（18）色谱柱（4.6 mm×150 mm,2.7μm）,流动相为0.1%甲酸（A）-甲醇（B）溶液,梯度洗脱,流速为0.3 m L/min,检测波长为370 nm,柱温为40℃。4-HD、XAG在0.945-18.900、1.048-20.960μg/m L质量浓度范围内线性关系良好,样品含量的相对标准偏差分别为3.13%、2.52%,方法的重复性好,平均回收率分别为103.70%、103.05%。经检测明日叶中4-HD的分布规律为：茎（149.16μg/g）〉根（122.42μg/g）〉叶（1.24μg/g）;XAG的分布规律为：根（144.28μg/g）〉茎（75.17μg/g）〉叶（1.21μg/g）。4-HD和XAG在根和茎中的分布显著高于其在叶中的分布。In this study, the contents of 4-hydroxyderricin（4-HD） and xanthoangelol（XAG） in roots, stems and leaves of Angelica keiskei were determined using high performance liquid chromatography with photo-diode array（HPLC-PDA）detector. The samples were separated on a Halo C（18）-column（4.6 mm × 150 mm, 2.7 μm）, gradiently eluted with 0.1% formic acid in water（A） and methanol（B） at a flow rate of 0.3 m L/min and detected at 370 nm. The column temperature was set at 40 ℃. Good linear relationships between peak area and injection amount of 4-HD and XAG were established within the range of 0.945–18.900 and 1.048–20.960 μg/m L, respectively. The relative standard deviations（RSDs） of peak area for the two compounds were 3.13% and 2.52%, respectively, suggesting good reproducibility of the developed method. The average recoveries of the spiked compounds were 103.70%, and 103.05%, respectively. The results showed that the distribution of 4-HD in different parts of Angelica keiskei was ranked in decreasing order as follows： stems（149.16 μg/g） roots（122.42 μg/g） 〉leaves（1.24 μg/g）, while the order of XAG content was as follows： roots（144.28 μg/g）〉 stems（75.17 μg/g）〉 leaves（1.21 μg/g）. The contents of 4-HD and XAG in roots and stems of Angelica keiskei were significantly higher than in leaves.

关 键 词：明日叶 4-羟基德里辛 黄色当归醇 高效液相色谱配备光电二极管阵列检测器 

分 类 号：TS207.3[轻工技术与工程—食品科学]