辅助降血糖保健食品水溶性多糖测定方法的研究  

Study on determination of the content of water- soluble polysaccharides in auxiliary hypoglycemic health food

作　　者：刘世娟[1] 张海弢[1] 王婧[1] 徐振秋[1] 

机构地区：[1]江苏康缘药业股份有限公司,江苏连云港222001

出　　处：《粮油食品科技》2016年第1期50-54,共5页Science and Technology of Cereals,Oils and Foods

摘　　要：建立一种辅助降血糖保健食品中水溶性多糖的测定方法,考察提取方式、醇沉浓度、脱蛋白方法及显色方法等因素对多糖测定结果的影响.结果表明选用苯酚硫酸法进行显色,超声提取60min,超声功率250W,醇沉浓度80%,以样液体积1/10倍量的5%三氯乙酸溶液,脱蛋白1次,静置时间2h,测定结果较好.葡萄糖浓度在6.61～79.28μg/mL范围内与吸光度有良好的线性关系,回归方程为A=0.0144C-0.0655（r=0.9996）,平均加标回收率为98.47%,RSD为1.64%.测得样品中水溶性多糖平均含量为11.49%,RSD为2.31%.该方法简便、准确,重复性好,可用于辅助降血糖保健食品中多糖含量的测定和质量控制.To establish a method for determination of the content of water - soluble polysaccharides in auxiliary hypoglycemic health food,the effects of extraction method,concentration of ethanol for precipi-tation,deproteinization method and coloration method on the determined values of polysaccharides content were studied.Result showed that the phenol -sulfuric acid method was chosen as the coloration method, with the conditions as follows:ultrasonic power 250 W,extraction time 60 min,80% ethanol concentra-tion for precipitation,and 1 /10 polysaccharide extraction volume,5% trichloroacetic acid concentration for deproteinization,deproteinization once,standing time 2 h,and the result was good.The standard curve regression equation was A =0.0144C -0.0655,the calibration curve had a good linearity in the range of 6.61 -79.28 μg/mL for glucose with the correlation coefficient (r)of 0.9996,and the average recovery at the spiked concentrations was 98.47% with RSD of 1.64%.The average content of water -soluble polysaccharides from the auxiliary hypoglycemic health food was 11.49% with RSD of 2.31%. This method was simple and accurate with a good repetion,which can be used to determine the content of polysaccharides in auxiliary hypoglycemic health products.

关 键 词：水溶性多糖 测定方法 苯酚硫酸法 保健食品 

分 类 号：TS201.4[轻工技术与工程—食品科学]