一种快速测定茶鲜叶中β-胡萝卜素的高效液相色谱方法  

A rapid determination method of β-Carotene in fresh tea leaves by high performance liquid chromatography

作　　者：李银花[1] 李娟[1] 邓婷婷[2] 吴扬[2] 

机构地区：[1]湖南农业大学茶学教育部重点实验室,长沙410128 [2]福建农林大学园艺学院,福州350002

出　　处：《食品安全质量检测学报》2016年第3期1024-1028,共5页Journal of Food Safety and Quality

基　　金：国家自然科学基金项目(31500567);湖南省重点学科建设项目~~

摘　　要：目的建立一种快速测定茶鲜叶中β-胡萝卜素的高效液相色谱(HPLC)方法。方法以丙酮为提取溶剂;色谱柱为SunFire^(TM)C_(18)(150 mm×4.6 mm,5μm);以三氯甲烷-乙腈溶液(9:1,V:V)为流动相,等梯度洗脱;波长450 nm;流速1.0 mL/min;柱温35℃。结果在该色谱条件下β-胡萝卜素与其他物质得到了有效的分离,且β-胡萝卜素在9.5×10^(-3)~0.76μg范围内与峰面积有良好的线性关系(r=0.99997),回收率97.32%~101.26%。结论该方法重复性好、精密度高、回收率较好,可作为茶鲜叶中β-胡萝卜素的定量测定方法。Objective To develop a determination method for β-Carotene in fresh tea by high performance liquid chromatography（HPLC）.Method The β-Carotene was extracted from fresh tea leaves by acetone.A SunFire^（？） C_（18） column（150 mm×4.6 mm,5 μm） was used with the mobile phase of chloroform-acetonitrile solution（9：1,V：V）.The flow rate was 1.0 mL/min,wavelength was 450 nm and the column temperature was35 ℃.Result This method showed a good linearity（r=0.99997） for β-Carotene（ranging from 9.5×10^（-3） to0.76 μg） and the recovery of β-Carotene was from 97.32%to 101.26%.Conclusion This method is precise,accurate and suitable for the determination of β-Carotene in fresh tea leaves.

关 键 词：高效液相色谱法 茶鲜叶 Β-胡萝卜素 

分 类 号：O657.72[理学—分析化学]