嗜热地衣芽孢杆菌植酸酶的原核表达及酶学性质研究  

Expression and characteristics of phytase from Bacillus licheniformis

作　　者：李平[1,2,3] 易弋[1,2,3] 石征宇[1,2,3] 伍时华[1,2,3] 黄翠姬[1,2,3] 黎娅[1,2,3] 

机构地区：[1]广西科技大学生物与化学工程学院,广西柳州545006 [2]广西糖资源绿色加工重点实验室(广西科技大学),广西柳州545006 [3]广西高校糖资源加工重点实验室(广西科技大学),广西柳州545006

出　　处：《食品工业科技》2016年第8期238-241,261,共5页Science and Technology of Food Industry

基　　金：广西科学基金(2014GXNSFAA118086);广西科学基金(2015GXNSFBA139068)

摘　　要：将地衣芽孢杆菌SR01(Bacillus licheniformis SR01)的植酸酶phy基因重组于表达载体p GEX-4T-3中,导入大肠杆菌BL21(Escherichia coli BL21)构建工程菌p GX1143,并得到了高效表达。对其酶学性质的研究表明:该酶最适反应p H为5.0,最适反应温度为55℃,具有广泛的p H稳定性和较好的热稳定性;Co^(2+))、Li^+、Mg^(2+))和Ba^(2+)对酶活性有促进作用,Cu^(2+)、Pb^(2+)、Fe^(2+)、Ag^+、SDS对酶活性有不同程度的抑制作用,并且Pb^(2+)、Fe^(2+)和SDS对酶活性的抑制作用较强烈;此外,该酶还具有非常好的抗胰蛋白酶水解能力和部分抗胃蛋白酶水解的能力。The gene phy,from a thermophilic bacteria strain Bacillus licheniformis SR01,was cloned into prokaryotic expression vector p GEX-4T-3 to construct the recombinant strain p GX1143,and it was successfully efficiently expressed. The optimal p H and temperature for this phytase activity were 5.0 and 55 ℃,respectively. The enzyme had a wide range of p H stability and good thermal stability. And it was activated by Co^（2＋）,Li~＋,Mg^（2＋）,Ba^（2＋）,and inhibited by Cu^（2＋）,Pb^（2＋）,Fe^（2＋）,Ag~＋,SDS. However,it almost lost activity when influenced by Pb^（2＋）,Fe^（2＋） and SDS. In addition,it had strong ability to resist hydrolyzation by trypsin and partial ability to resist hydrolyzation by pepsin.

关 键 词：地衣芽孢杆菌 植酸酶 基因克隆 酶学性质 

分 类 号：Q939.124[生物学—微生物学]