基于涂膜法FTIR的食用油羰基值快速检测  

Rapid Fourier Transform Infrared Spectroscopic(FTIR)Determination of Carbonyl Value in Edible Oils Using Polyethylene Film

作　　者：李易文[1] 陈倩[1] 张鹏[1] 李晶[1] 张睿[1] 于修烛[1] 

机构地区：[1]西北农林科技大学食品科学与工程学院,陕西杨凌712100

出　　处：《食品科学》2016年第8期226-230,共5页Food Science

基　　金：西北农林科技大学国家级大学生创新训练项目(201410712076)

摘　　要：为了建立食用油羰基值快速检测方法,以菜籽油为实验材料,茴香醛为羰基标准物配制系列羰基值(0~60 mmol/kg)油样。用涂膜法采集油样光谱,建立羰基值与特征吸收峰1 703/1 570 cm-1处(1 703 cm^(-1)处峰高两点基线均为1 570 cm^(-1),下同)吸光度的关系。然而,不同种类零羰基值的食用油在特征吸收峰1 703 cm^(-1)处的吸收不相同而引起基底效应。因此,利用二维相关振动光谱技术寻找特征吸收峰的相关吸收峰,以校正食用油间的基底效应。结果表明:油样在1 703 cm^(-1)处的吸光度与羰基值高度线性相关,利用相关吸收峰3 472 cm^(-1)校正后的模型为y=0.005 4x+0.090 9;相关系数(R)为0.991 9,标准偏差(s)为0.013 mmol/kg。模型预测值与国标法测定值高度线性相关,所得方程的斜率接近于1,R值均在0.99以上,两种方法测定结果十分接近。表明涂膜法傅里叶变换红外光谱检测食用油羰基值是可行的。This study aimed to develop a rapid method for the detection of carbonyl value（CV）in edible oils.Anisaldehyde was used as carbonyl reference and gravimetrically added to carbonyl-free rapeseed oil.A series of CV（0–60 mmol/kg）oil samples were determined using polyethylene（PE）film as a spectrum acquisition accessory.A linear relationship between CV and absorbance at the characteristic absorption peak（1 703/1 570 cm^（-1）,peak at 1 703 cm^（-1） and two base points at1 570 cm^（-1））was established.However,different types of edible oils exhibited substrate effect,which showed that 1 703 cm^（-1） had variability even among their carbonyl-free samples.To correct the substrate effect,peak absorption at 3 472 cm^（-1） ,as evaluated by the 2-D correlation spectroscopy technique,was used as the relevant absorption peak to 1 703 cm^（-1） .Subsequently,a liner relationship between both peak heights was established.Results indicated that there was a highly linear correlation between CV and the absorbance at 1 703 cm^（-1） .The corrected model was y = 0.005 4 x ＋ 0.090 9.with a correlation coefficient（R）of 0.991 9 and standard deviation of 0.013 mmol/kg.Moreover,there was a good relationship between the values obtained from the national standard method and the corrected model,with a slope close to 1,and correlation coefficient of more than 0.99.Thus the PE film-based FTIR method for determination CV in edible oils is workable.

关 键 词：食用油 傅里叶变换红外光谱 羰基值 检测 

分 类 号：TS227[轻工技术与工程—粮食、油脂及植物蛋白工程]