禾谷镰刀菌发酵液中玉米赤霉烯酮毒素的提取及纯化研究  

Extraction and purification of zearalenone in Fusarium graminearum

作　　者：甄玉萍 裴世春[1] 高建伟[1] 王岩[1] 郝广 

机构地区：[1]齐齐哈尔大学食品与生物工程学院,黑龙江齐齐哈尔161006

出　　处：《食品工业科技》2016年第9期155-158,164,共5页Science and Technology of Food Industry

基　　金：齐齐哈尔大学研究生创新科研项目(YJSCX2014-015X);国家科技基础性工作专项(2013FY113400);黑龙江省教育厅科学计划项目(12541871);齐齐哈尔大学启动基金(000240)

摘　　要：本研究旨在通过不同萃取剂和层析柱的使用,从禾谷镰刀菌发酵液中提取、纯化玉米赤霉烯酮(zearalenone,ZEN)毒素,以期获得较高纯度的毒素。采用超高效液相色谱串联质谱(ultra-performance liquid chromatography-mass spectrometry,UPLC-MS)分析方法对其效果进行检测。研究结果表明:在单一的萃取剂中,苯的萃取效果最佳,其得率可达到73.20%;采用Daisogel反相层析柱进行纯化,得率可达到69.30%,且样品中的杂峰数目明显减少,由此证明该方法对毒素纯化效果较好。本研究对发展高纯度ZEN毒素的制备技术具有一定的指导意义和参考价值。To obtain highly purified zearalenone,the mycotoxin was extracted and then purified from the fermented liquid of Fusarium graminearum in this study using different extraction solvents and chromatographic columns.The effects of extraction and purification were evaluated by ultra- performance liquid chromatography- mass spectrometry（ UPLC- MS）.The results demonstrated that benzene was recognized to be the best one of the single extraction agents,ewhose yield could reach up to 73.20%.Daisogel reverse phase chromatography column was much better chosen to purify zearalenone because its yield was up to 69.30% and the numbers of the impurity peaks significantly decreased.The findings of this study can provide the guidance and reference for the development of highly purity of zearalenone.

关 键 词：真菌毒素 玉米赤霉烯酮 禾谷镰刀菌 提取 纯化 

分 类 号：TS201.3[轻工技术与工程—食品科学]