β-葡萄糖苷酶粗酶液的超滤条件研究  

Study on ultrafiltration conditions for β- glucosidase crude enzyme liquid

作　　者：冯玉枚 肖玲玲[2] 郭金玲[1] 吕育财[1] 余华顺[3] 姚鹃[3] 龚大春[1] 

机构地区：[1]三峡大学艾伦麦克德尔米德再生能源研究所,湖北宜昌443002 [2]湖北三峡职业技术学院,湖北宜昌443002 [3]安琪酵母股份有限公司酵母功能湖北省重点实验室,湖北宜昌443002

出　　处：《食品工业科技》2016年第9期165-168,174,共5页Science and Technology of Food Industry

基　　金：国家自然基金面上项目(21076114);宜昌市科技攻关项目(A15-101)

摘　　要：采用超滤技术分离纯化β-葡萄糖苷酶粗酶液,以膜通量、回收率和比酶活为综合评价指标,筛选出合适的截留分子量的膜材料,并进一步考察了进口压力、回流压力、粗酶液固形物含量、膜过滤时间等因素对β-葡萄糖苷酶粗酶液超滤浓缩的影响。结果表明,β-葡萄糖苷酶粗酶液最佳超滤工艺条件为:选用50 ku的超滤膜,对0.9%的料液浓度在进口压0.12 MPa、回流压0.08 MPa条件下超滤20 min,浓缩倍数达3倍,β-葡萄糖苷酶回收率达95.05%,比酶活提高1.24倍,平均膜通量达96.0 L/(m^2·h)。用超滤膜浓缩β-葡萄糖苷酶具有工艺简单、能耗低、绿色环保、β-葡萄糖苷酶活性损失小等优点,为β-葡萄糖苷酶的工业化生产放大奠定基础。β- Glucosidase crude enzyme liquid by ultrafiltration was purified.The appropriate molecular weight cutoff of the ultrafiltration membrane was screened by taking the membrane flux,recovery and specific activity as comprehensive indexes.Then the effects of inlet pressure,outlet pressure,crude enzyme liquid solid content onβ- glucosidase ultrafiltration were studied.The results showed the optimal conditions were as follows： under these conditions of molecular weight cutoff of ultrafiltration membrane 50 ku,0.9% feed concentration of crude enzyme solution,inlet pressure 0.12 MPa,outlet pressure 0.08 MPa,operate time 20 min,β- glucosidase crude enzyme liquid was concentrated by three times,the specific activity of β- glucosidase was increased by 1.24 times with95.05% total recovery,and the average membrane flux can reach 96.0 L /（ m2·h）.Ultrafiltration membrane process for purification β- glucosidase was simple and the purified β- glucosidase can remain high activity,thus this method was suitable for industrial production with many advantages of good economic and social benefits and low energy consumption,low cost and no environmental pollution.

关 键 词：Β-葡萄糖苷酶 超滤 浓缩 比酶活 

分 类 号：TS205.4[轻工技术与工程—食品科学]