粪便微生物粗提液修复溃疡性结肠炎的研究  

Recovery effect of fecal microbial extracts on mouse with ulcerative colitis

作　　者：徐敏[1] 边鑫[1] 杜金城[1] 丁秀云[1] 于上富 霍贵成[1] 

机构地区：[1]东北农业大学,乳品科学教育部重点实验室,黑龙江哈尔滨150030

出　　处：《食品工业科技》2016年第9期367-371,共5页Science and Technology of Food Industry

基　　金：国家自然科学基金(31401512);国家863项目:乳酸菌特色资源库及乳酸菌发酵剂和代谢工程技术研究(2011AA100902)

摘　　要：研究了粪便微生物粗提液对溃疡性结肠炎修复作用的影响。将64只健康小鼠随机分为4组,即完全空白组(n=20)、肠炎空白组(n=20)、SASP(柳氮硫胺吡啶)组(n=12)和FMT(粪便微生物移植)组(n=12),处理组小鼠自由饮用3%葡聚糖硫酸钠盐(DSS)溶液8 d,建立溃疡性结肠炎(UC)模型,并于建模第8 d,解剖完全空白组、肠炎空白组小鼠各8只,以验证UC模型是否建立成功。造模后,SASP组、FMT组同时分别给予柳氮硫胺吡啶片及鼠源粪便微生物提取液干预治疗4周,灌胃频率2 d/次,然后进行为期两周的后续观察,期间观察各组小鼠表观状态、体重变化,结肠组织长度变化,结肠组织病理学改变,并测定各组血清IL-1β、IL-6、TNF-α的浓度变化。结果表明:与肠炎空白组小鼠相比,粪便微生物移植疗法使小鼠血清IL-1β、IL-6、TNF-α水平显著降低(p<0.05),并使结肠长度及体重显著增加,结肠组织更加完整。因此证实,粪便微生物提取液对溃疡性结肠炎具有较好的治疗作用,具有应用于临床医学的潜力。The recovery effect of fecal microbial extracts on ulcerative colitis mouse was studied.64 healthy mice were randomly divided into 4 groups,which was the sham group（ n = 20）,the negative control group（ n = 20）,the positive control group（ n = 12）,and the FMT group（ n = 12）.The mice from the treated groups were freely drunken3% dextran sulfate sodium salt（ DSS） solution for 8 days to establish the ulcerative colitis（ UC） model,and 8 mice from the sham group and 8 mice from the negative group were died to verify whether the UC model was set successfully at the 8- th day.After the UC model,the SASP group mice were treated with the SASP solution for four weeks,2 days / time,and the FMT group mice were treated with the fecal microbial extracts from the healthy mouse for four weeks,2 days / time.After that,the follow- up of the mice was studied for two weeks,to observe the mouse state,the weight,the colon length,the colon organization pathology and the serum IL-1β,and IL-6,and TNF-α.Results showed that the serum levels of IL- 6,IL- 1β and TNF- α were significantly decreased in fecal microbial extracts group（ p〈 0.05）,compared with the negative group,the length and weight of the colon were significantly increased in fecal microbial extracts group,and the colon was made more complete.Thus,stool microbial extracts had good effect on the ulcerative colitis and have the potential to be applied into the clinical medicine.

关 键 词：粪便微生物粗提液 溃疡性结肠炎 结肠 血清 

分 类 号：TS201.4[轻工技术与工程—食品科学]