克雷伯氏菌产1,3-丙二醇aldH基因缺失菌株的构建  

Construction of 1,3-propanediol-producing Klebsiella pneumoniae strain with aldH gene deletion

作　　者：杨建楼 郑海杰[1] 马春玲[1] 王瑞明[1] 陈利飞 

机构地区：[1]齐鲁工业大学食品与生物工程学院,山东省微生物工程重点实验室,山东济南250353 [2]山东华牧天元农牧股份有限公司,山东济南250353

出　　处：《中国酿造》2016年第4期56-60,共5页China Brewing

基　　金：山东省自主创新及成果转化专项(201422cx02602);泰山学者建设工程专项经费资助

摘　　要：该研究利用λRed重组技术对克雷伯氏菌(Klebsiella pneumonia)中的乙醛脱氢酶(ald H)基因进行敲除,成功构建缺失菌株K.pneumonia2-1Δald H,与原始菌株相比,缺失菌株K.pneumonia2-1Δald H发酵液中乙醇产量由原来的7.24 g/L降为0.47 g/L,降低了93.51%;1,3-丙二醇产量由原来的78.83 g/L增长至82.55 g/L,提高了4.72.%;甘油转化率由60.64%增长至63.50%,提高了2.86%。缺失菌株K.pneumonia2-1Δald H 50 L发酵罐小试试验中,1,3-丙二醇的产量为78.13 g/L,乙醇产量为0.50 g/L。The aldehyde dehydrogenase （aldH） gene in Klebsiella pneumoniae was deleted by λRed recombinant technology, and the disruptant strain K. pneumonia 2-1ΔaldH was established successfully. Compared with the original strain, the ethanol yield in the disruptant strain fermentation liquor reduced from 7.24 g/L to 0.47 g/L, which reduced by 93.51%. The 1,3-propanediol yield increased from 78.83 g/L to 82.55 g/L, which increased by 4.72%, and the glycerinum conversion rate increased from 60.64% to 63.50%, which increased by 2.86%. In 50 L fermentation tank experiments of the disruptant strain, the 1,3-propanediol yield was 78.13 g/L, and the ethanol yield was 0.50 g/L.

关 键 词：克雷伯氏菌 1 3-丙二醇 aldH基因 λRed同源重组 

分 类 号：TQ923[轻工技术与工程—发酵工程]